After the data has been reviewed and bad cells have been removed from the data set, the results of the analysis can be exported to an Excel file. This step will function on all platforms, and will result in an Excel file with a separate worksheet for each genotype. Each worksheet will contain the fitted parameters, per sucrose pulse (´block´), of all analysed files for this genotype, sorted by sucrose concentration. The naming convention for these worksheets is "par_[genotype]" (e.g. "par_WT"). Furthermore, an 'Info' sheet is generated, which contains information on the raw data. Further analysis, such as calculation of mean and SEM values, as well as testing for statistical significance can be done in Excel.
Typically, the structure of one worksheet in such an exported file is as follows:
Block | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
File name | Sucrose concentration (mM) | Priming rate k1 (1/s) | Unpriming rate k-1 (1/s) | Release rate constant k2max (1/s) | Onset delay tdel (s) | Rise time tau (s) | Depot pool D (pC) | Primed pool R (pC) | Cumulative release (pC) | Cumulative refill (pC) |
The leftmost column contains the file names, while the next column gives the sucrose concentration used in each of these recordings. All other columns contain the fitted parameters for a single sucrose pulse. Submaximal concentrations furthermore contain columns describing the cumulative release and the cumulative refill obtained from the fits. These values can be used to calculate the fraction of RRP depleted by the submaximal stimulus. For each subsequent sucrose pulse ('block'), all these columns - except for the ones containing the file names and sucrose concentrations - are repeated (with different fitted values, obviously) and headed by a 'Block' cell.
This data sheet contains a summary of the raw data properties per data file, as well as the fit quality. Its structure is typically as follows:
Block | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
File name | Sucrose concentration (mM) | Genotype | Fitting method | Fit start (s) | Sucrose pulse (s) | Baseline current (pA) | Baseline slope (pA/s) | Noise level (pA) | Peak current (pA) | Fit quality (pA) |
For each subsequent sucrose pulse ('block'), all these columns - except for the ones containing the file names, sucrose concentrations and genotypes - are repeated and headed by a 'Block' cell.