Activity assays were performed by dephosphorylating synthetic phosphopeptides using recombinant PP1 enzyme or PP1-Phactr1 or PP1-Neurabin fusions.

The summary_of_all_activity_assays.xlsx file contains measurements from 6 independent experiments. 
Summary data is organised in tabs: catalytic efficiency, Michaelis constant, and a tab for each independent replicate. 

The folder 'raw_data_activity_assays' contains raw readout data from a plate-reader and initial processing, such as conversion of RFU to phosphate concentration, normalisation and curve fitting. 
Each independent replicate measurement is based on a curve fit through for kinetic rates at several peptide concentrations, while rate measurement at each peptide concentration is done in 4 replicates.