FIMO Results

FIMO - Motif search tool

FIMO version 5.5.5, (Release date: Thu Sep 14 08:48:04 2023 +1000)

For further information on how to interpret these results please access https://meme-suite.org/meme/doc/fimo-output-format.html.
To get a copy of the FIMO software please access https://meme-suite.org

If you use FIMO in your research, please cite the following paper:
Charles E. Grant, Timothy L. Bailey, and William Stafford Noble, "FIMO: Scanning for occurrences of a given motif", Bioinformatics, 27(7):1017-1018, 2011. [full text]

DATABASE AND MOTIFS

  DATABASE cluster_4_coord_nodup.txt.fa
  
  Database contains 462 sequences, 13860 residues

  MOTIFS streme_out/streme.xml (DNA)
  
        MOTIF
        WIDTH
        
         BEST POSSIBLE MATCH
        
        1-CAGGMA
        6
        CAGGCA
       
        2-CCACAGA
        7
        CCACAGA
       
        3-ATGAYTCA
        8
        ATGATTCA
       
        4-GTGAGCT
        7
        GTGAGCT
       
Random model letter frequencies (./background):

A 0.279 C 0.221 G 0.221 T 0.279

MOTIF	WIDTH	BEST POSSIBLE MATCH
1-CAGGMA	6	CAGGCA
2-CCACAGA	7	CCACAGA
3-ATGAYTCA	8	ATGATTCA
4-GTGAGCT	7	GTGAGCT

SECTION I: HIGH-SCORING MOTIF OCCURENCES

There were 23 motif occurences with a p-value less than 0.0001. The full set of motif occurences can be seen in the TSV (tab-delimited values) output file fimo.tsv, the GFF3 file fimo.gff which may be suitable for uploading to the UCSC Genome Table Browser (assuming the FASTA input sequences included genomic coordinates in UCSC or Galaxy format), or the XML file fimo.xml.
The p-value of a motif occurrence is defined as the probability of a random sequence of the same length as the motif matching that position of the sequence with as good or better a score.
The score for the match of a position in a sequence to a motif is computed by summing the appropriate entries from each column of the position-dependent scoring matrix that represents the motif.
The q-value of a motif occurrence is defined as the false discovery rate if the occurrence is accepted as significant.
The table is sorted by increasing p-value.

Motif ID	Alt ID	Sequence Name	Strand	Start	End	p-value	q-value	Matched Sequence
3-ATGAYTCA	STREME-3	chr20	+	58342467	58342474	2.32e-05	0.0602	ATGATTCA
3-ATGAYTCA	STREME-3	chr11	+	125128624	125128631	2.32e-05	0.0602	atgattca
3-ATGAYTCA	STREME-3	chr1	+	183608473	183608480	2.32e-05	0.0602	atgattca
3-ATGAYTCA	STREME-3	chr7	+	35762506	35762513	4.15e-05	0.0602	ATGACTCA
3-ATGAYTCA	STREME-3	chr22	+	38178201	38178208	4.15e-05	0.0602	ATGactca
3-ATGAYTCA	STREME-3	chr7	+	73774086	73774093	4.15e-05	0.0602	atgactca
3-ATGAYTCA	STREME-3	chr11	+	105719642	105719649	4.15e-05	0.0602	ATGACTCA
3-ATGAYTCA	STREME-3	chr10	+	110188673	110188680	4.15e-05	0.0602	atgactca
3-ATGAYTCA	STREME-3	chr11	+	119166504	119166511	4.15e-05	0.0602	ATGACTCA
3-ATGAYTCA	STREME-3	chr2	+	181791355	181791362	4.15e-05	0.0602	ATGACTCA
3-ATGAYTCA	STREME-3	chr2	+	230687818	230687825	4.15e-05	0.0602	ATGACTCA
3-ATGAYTCA	STREME-3	chr22	+	26929750	26929757	5.98e-05	0.0602	atgagtca
3-ATGAYTCA	STREME-3	chr2	-	28617614	28617621	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr7	-	35762507	35762514	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr11	-	75558314	75558321	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr11	+	90483791	90483798	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr11	-	105719643	105719650	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr10	-	110188674	110188681	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr6	-	131789337	131789344	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr3	+	143135240	143135247	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr1	-	172325778	172325785	5.98e-05	0.0602	ATGAGTCA
3-ATGAYTCA	STREME-3	chr12	-	59399367	59399374	8.91e-05	0.082	ATGATTAA
3-ATGAYTCA	STREME-3	chr5	+	120803510	120803517	8.91e-05	0.082	ATGATTaa

DEBUGGING INFORMATION

Command line:

fimo --verbosity 1 --oc fimo_out_5 --bgfile ./background --motif 3-ATGAYTCA streme_out/streme.xml cluster_4_coord_nodup.txt.fa

Settings:


  
    output_directory = fimo_out_5
    MEME file name = streme_out/streme.xml
    sequence file name = cluster_4_coord_nodup.txt.fa
  
  
    background file name = ./background
    alphabet = DNA
    max stored scores = 100000
  
  
    allow clobber = true
    compute q-values = true
    parse genomic coord. = true
  
  
    text only = false
    scan both strands = true
    max strand = false
  
  
    threshold type = p-value
    output theshold = 0.0001
    pseudocount = 0.1
  
  
    alpha = 1
    verbosity = 1

This information can be useful in the event you wish to report a problem with the FIMO software.

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output_directory = fimo_out_5	MEME file name = streme_out/streme.xml	sequence file name = cluster_4_coord_nodup.txt.fa
background file name = ./background	alphabet = DNA	max stored scores = 100000
allow clobber = true	compute q-values = true	parse genomic coord. = true
text only = false	scan both strands = true	max strand = false
threshold type = p-value	output theshold = 0.0001	pseudocount = 0.1
alpha = 1	verbosity = 1