Acquisition of genes through horizontal gene transfer (HGT) allows microbes to rapidly gain new capabilities and adapt to new or changing environments. Identifying widespread HGT regions within multispecies microbiomes can pinpoint the molecular mechanisms that play key roles in microbiome assembly. We sought to identify horizontally transferred genes within a model microbiome, the cheese rind. Comparing 31 newly sequenced and 134 previously sequenced bacterial isolates from cheese rinds, we identified over 200 putative horizontally transferred genomic regions containing 4733 protein coding genes. The largest of these regions are enriched for genes involved in siderophore acquisition, and are widely distributed in cheese rinds in both Europe and the US. These results suggest that HGT is prevalent in cheese rind microbiomes, and that identification of genes that are frequently transferred in a particular environment may provide insight into the selective forces shaping microbial communities.
DOI:http://dx.doi.org/10.7554/eLife.22144.001
10.7554/eLife.22144.002eLife digest
From the depths of the ocean to the lining of the human gut, almost every environment on Earth is home to a unique community of microorganisms referred to as a microbiome. Within these communities, unrelated microorganisms can exchange genetic information through a process known as horizontal gene transfer. For example, genes linked to antibiotic resistance are often transferred between different microorganisms, which can create increasingly drug resistant microbes and has important implications for human health.
Horizontal gene transfer has been studied for almost 100 years, but examining it directly is challenging because, almost by definition, it requires studying a community of microbes rather than one microbe in isolation. As such, researchers are looking for simple models of microbial communities that can be easily manipulated in experiments.
Bonham et al. have now turned to the outer surface of cheese, also known as cheese rind, to better understand horizontal gene transfer. As a model system, the cheese rind microbiome is relatively simple to work with because cheese rind is easy to replicate in the laboratory, and the microbes growing on cheese can be grown on their own or in combinations with other microbes. By comparing the genetic material of 165 cheese-associated bacteria to one another, Bonham et al. identified over 4,000 genes that were shared between the bacteria, including several large clusters of genes that were shared by many species.
Many of the identified genes (about 23% to be precise) appear to help the microorganisms acquire nutrients that are known to be in short supply on the surface of cheese surface, including iron. Bacteria typically use specialized molecules called siderophores to scavenge for iron and uptake systems to carry the iron-bound siderophore back into the cell. Notably, only the genes associated with the uptake systems were found in some of the shared gene clusters. This finding suggests that horizontal gene transfer has allowed some microbes to “cheat” and take up iron-bound siderophores without expending energy to produce the siderophores themselves.
Using the cheese rind microbiome as a model system, it becomes possible to explore how horizontal gene transfer works in more detail than before. A better understanding of this process can then be applied to other important microbiomes, including those where genes conferring antibiotic resistance are commonly exchanged.
DOI:http://dx.doi.org/10.7554/eLife.22144.002
Author KeywordsMicrobial communitiesCheeseHorizontal Gene TransferSiderophoresModel systems for microbial communitiesResearch OrganismOtherhttp://dx.doi.org/10.13039/100000002National Institutes of HealthP50 GM068763BonhamKevin SWolfeBenjamin EDuttonRachel JThe funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.elife-xml-version2.5Author impact statementThe identification of horizontally transferred genes, and commonly transferred functions, can provide a window into the selective forces acting on species within an ecosystem.eLife Digest2.5Introduction
Great strides have been made in characterizing the composition of microbiomes, and in understanding their importance in the ecology of natural systems, in agriculture and in human health. However, despite these advances, the forces that shape the diversity, structure, and function of microbiomes remain poorly understood (Widder et al., 2016). Investigating these underlying mechanisms in situ is difficult, as observational and sequenced-based analysis rarely enables causal conclusions (Nemergut et al., 2013). Replicating microbial communities in vitro is also an enormous challenge, because of high levels of diversity and the difficulties in establishing pure cultures of most bacterial species. These obstacles significantly hamper our ability to move from observations of microbial diversity to the molecular mechanisms shaping key processes such as species interactions and microbial evolution.
Horizontal gene transfer (HGT) is a major force in microbial evolution and can lead to the wholesale acquisition of novel functions. In some cases, these novel functions can have significant adaptive consequences, such as in the transfer of antibiotic resistance genes (Ochman et al., 2000). HGT also allows rapid adaptation to new niches (Wiedenbeck and Cohan, 2011), as ecologically relevant genes may be acquired by species not previously adapted to a particular niche (Tasse et al., 2010; Hehemann et al., 2010). The movement of microbes to new environments has been shown to increase both the rate and impact of HGT, and HGT is most frequent for genes under positive selection (Niehus et al., 2015). In moving to a new environment, microbes can face novel abiotic conditions (temperature, moisture, salinity, pH, and nutrients) and novel biotic challenges and opportunities resulting from the presence of microbial neighbors.
Evaluating HGT within the context of microbial communities could provide new insights concerning the extent, mechanisms, and ecological impact of this important process. Advances in genome sequencing have begun to provide a glimpse into HGT within environmentally, medically and economically important microbiomes (McDaniel et al., 2010; Andam et al., 2015). For example, extensive gene sharing has been observed throughout the commensal human microbiome, including sharing of genes that enable nutrient acquisition from novel food sources (Hehemann et al., 2010; Smillie et al., 2011), and pathogenicity islands and antibiotic resistance genes in pathogenic microbes (McCarthy et al., 2014; Hiramatsu et al., 2001; Forsberg et al., 2012). There is evidence of extensive HGT in other natural habitats, such as soil (Coombs and Barkay, 2004; Heuer and Smalla, 2012) and aquatic environments (McDaniel et al., 2010; Frischer et al., 1994). Although these studies offer valuable insights into the frequency and potential impact of genes that can be transferred in microbial communities, the complexity of these systems makes difficult any further examination of the effects of these HGT events on their evolution and ecology.
The microbial communities of fermented foods experience strong selection as a result of growing in novel, human-made environments. Previous work has demonstrated that HGT can be a major driver of adaptation in food systems and other human-managed environments (Andam et al., 2015; Rossi et al., 2014). Prior analysis of microbial species from cheese has revealed several instances of HGT in this environment. Lactic acid bacteria (LAB) such as Lactobacillus and Lactococcus, which are used in the initial fermentation of milk, are known to harbor antibiotic resistance genes and may be reservoirs for transfer to pathogenic enterococci (Wang et al., 2006; Mathur and Singh, 2005) and other pathogenic microbes. Other food-associated bacteria may also contribute to antibiotic resistance gene transfer (Cocconcelli et al., 2003; Delorme, 2008; Flórez et al., 2005). In yogurt, another dairy ferment using LAB, HGT of metabolic genes has been observed between protocooperative species L. bulgaricus and S. thermophilus (Li et al., 2013; Liu et al., 2009). Sequencing of Penicillium species isolated from the cheesemaking environment identified HGT of large genomic islands between these key fungal inhabitants of cheese (Cheeseman et al., 2014; Ropars et al., 2015; Gibbons and Rinker, 2015).
During the aging of traditional styles of cheese in caves or aging rooms, bacteria and fungi form a multi-species biofilm called the rind (Button and Dutton, 2012). We have previously shown that these communities can be used to examine community-based processes, such as succession and interspecies interactions, within an experimentally tractable system (Wolfe et al., 2014; Kastman et al., 2016). Given that biofilms such as these are densely populated, and microbes in cheese rinds are under strong selection to obtain limited nutrients (e.g. free amino acids, iron) as well as tolerate cell stress (Monnet et al., 2015), we predicted that HGT might be widespread in cheese rind microbiomes and therefore might provide a useful experimental model for HGT within microbial communities.
We sought to determine the diversity, distribution, and functional content of HGT in bacterial species isolated from cheese rinds. Specifically, we predicted that (1) HGT would be widespread, (2) HGT genes would be enriched for functions related to survival in cheese environment, and (3) there would be uneven distribution of HGT events across taxa. We analyzed the genomes of newly isolated and sequenced cheese-associated bacterial species (31 genomes) and those available in public databases (134 additional genomes). We present data which suggest that there is extensive HGT in cheese-associated bacteria. The regions of DNA identified appear to encode a number of functions which would be expected to provide adaptive advantages within the cheese environment. In particular, we identified three large multi-gene islands that are shared within multiple Actinobacteria, Proteobacteria, and Firmicutes species. These genomic regions are not related, but appear to have analogous functions involving iron acquisition, and are widely distributed in geographically distant cheeses. This work provides foundational knowledge in an experimentally tractable system in which future work could help to provide insight into the role of HGT within microbiomes.
ResultsIdentification of putative horizontally transferred regions
To establish a diverse database of cheese-associated bacterial genomes, we isolated species from cheese samples collected as part of previous work (Wolfe et al., 2014). A total of 31 isolates, representing four bacterial phyla and 11 genera, were selected for genome sequencing using Illumina and PacBio (Supplementary file 1a). Recently, a large collection of cheese-associated bacterial genomes were sequenced (Almeida et al., 2014), allowing inclusion of additional genomes in our analysis. Our isolates were from cheeses produced in the United States, Spain, Italy, and France, while the Almeida et al. collection was almost exclusively from France. We also included genomes from the NCBI reference sequence (RefSeq) database that are associated with cheese, for a total of 165 bacterial genomes.
We next developed a computational pipeline for identification of putative horizontally transferred genes, adapted from work on the human microbiome (Smillie et al., 2011). We built a central BLAST database containing all ORFs from all cheese-associated genomes. For each gene in each genome, we performed BLAST against this database, and compiled a list of hits (Figure 1—figure supplement 1, Materials and methods). For each hit, we examined the length and percent identity of aligned regions. Closely related species will have many nearly identical genes as a result of vertical inheritance. To avoid capturing these genes in our analysis, we determined the pairwise average nucleotide identity (ANI) between species within the same genus (Chan et al., 2012; Rodriguez-R and Konstantinidis, 2016). ANI provides a measure of the overall similarity of two genomes. We tested varying thresholds for length and ANI to examine the effects of these parameters on the results (Supplementary file 1c). Higher maximum ANI cutoffs and shorter lengths are more likely to yield false positives, as closely related species are more likely to share short stretches of high nucleotide identity. At the same time, a lower maximum ANI cutoff may exclude legitimate HGT events, especially considering that closely related species are also more likely to engage in HGT. Based on our most conservative gene identity parameters (minimum 99% identity over 500 nucleotides), we identified at least one putative horizontally transferred gene in 130/165 (78.8%) cheese-associated species in the analysis, for a total of 4733 genes (Figure 1A, Supplementary file 1d). Because this analysis depends on the species included for comparison, this list of HGT is almost certainly an underestimate.10.7554/eLife.22144.003
Extensive horizontal gene transfer in the cheese microbiome.
(A) All HGT events in analyzed cheese-associated bacteria. Connection thickness is scaled to number of shared protein coding sequences. Maximum likelihood tree based on 16S RNA alignment using Ribosomal Database Project (RDP). (B) HGT events clustered into 264 ‘groups’ based on genomic proximity. Groups are plotted based on total nucleotide content (x-axis, from low to high), and the mean number of genes per species (y-axis). Diameter of each circle is proportional to the total number of species in the group. Groups highlighted in red are described further in the text. (C) Quantification of KEGG modules and submodules for protein coding genes (CDS) identified as horizontally transferred. Annotations were generated by BLAST-Koala. Genes without function prediction are not depicted.
DOI:http://dx.doi.org/10.7554/eLife.22144.003
10.7554/eLife.22144.004
Schematic of software pipeline to identify HGT.
(1) Sequenced genomes are annotated with IMG/ER and downloaded in Genbank format. (2) All annotated genes in all genomes are used to assemble a BLAST database using BLAST+ command-line tools. (3) All protein coding genes (CDS) from all species are queried against the BLAST database. Hits from the same species are discarded; hits from species with an ANI > 89% are discarded; other hits are saved. (4) For each species, coding sequences that have at least one BLAST hit are grouped into islands based on proximity. Genes that are within 5 kb of each other on the same contig are considered to be part of the same island. (5) Islands in each species are compared with islands in other species to form groups. Islands that share at least one gene in common according to BLAST parameters in step 3 are placed in the same group.
DOI:http://dx.doi.org/10.7554/eLife.22144.004
10.7554/eLife.22144.005
Same as <xref ref-type="fig" rid="fig1">Figure 1A</xref> with branch labels.
All HGT events in analyzed cheese-associated bacteria. Connection thickness is scaled to # of shared protein coding sequences. Phylogenetic tree based on 16S RNA alignment using Ribosomal Database Project (RDP).
DOI:http://dx.doi.org/10.7554/eLife.22144.005
As multiple genes can be transferred in a single HGT event, we next assembled the putative HGT genes into groups based on genomic proximity. Individual coding sequences (CDS) for each species were grouped into islands if they were found within 5000 nucleotides of one another on the same contig. These islands were then clustered with islands in other species that shared at least one CDS. The 4733 genes were clustered into 264 individual groups (Figure 1B, Supplementary file 1d). Mobile elements such as transposons complicate our method of group clustering, as non-contiguous islands may be grouped together if they share a common transposon. Indeed, this appears to have occurred with Group 1, which contains genes from several disparate genomic regions. In other cases, a single species may have genes in a single group spread across multiple contigs (Figure 2—figure supplement 1), but this may accurately represent a single HGT event.
Most HGT groups identified (231, 87.5%) contain only members of the same phylum, or even a single genus (183 or 69%, Supplementary file 1d). This supports previous studies which suggest that HGT is most prevalent among closely related species (Ravenhall et al., 2015). However, we uncovered several notable exceptions. For example, Alkaibacterium kapii FAM208.38, a Firmicute, has a substantial (~8 kb) fraction of Group 1, which is predominantly found in Actinobacteria species. Groups 2 and 3 each have hits found in three different phyla (although both are predominantly found in Actinobacteria).
Functions encoded in HGT regions
HGT can enable rapid evolution of microbes entering a new environment, with genes that are under positive selection observed more frequently (Wiedenbeck and Cohan, 2011). Identifying the functions of genes that are frequently transferred could help to identify the selective forces that are most important for adapting to the cheese rind environment. Across all of the genes identified in our analysis, the most abundant gene functions are transposases, conjugal transfer genes, phage-related proteins, and other mobile elements (631/4733 or 13% of all protein coding sequences). A third (86/264 or 32.6%) of all HGT groups contain mobile elements. These genetic elements are likely involved either directly or indirectly in mobilization and transfer of DNA.
To determine whether gene functions other than mobile elements are enriched in identified HGT regions, we used BlastKOALA (Kanehisa et al., 2016) to assign KEGG functional annotations (Figure 1C, Figure 2—figure supplement 1c). In approximately half (53%) of the genes, KEGG annotations could not be assigned. Of the KEGG-annotated genes, the most frequent module (281/2264 or 11%) was ‘metal ion, iron siderophore and vitamin B12 transport systems’. Five of the 10 largest HGT groups as measured by total number of genes (Groups 1, 2, 3, 7 and 8) contained siderophore transport systems (K02013-K02016). Low availability of iron in cheese is known to limit the growth of several bacterial species (Monnet et al., 2012, 2010). Previous work has also shown that genes involved in iron acquisition are present in higher numbers in cheese-associated species compared with closely related species from other environments (Monnet et al., 2010; Walter et al., 2014).
Many other horizontally transferred genes (267/2264 or 12% of KEGG-annotated genes) are also involved in transport of nutrients relevant for growth in the cheese environment. Lactate is an abundant carbon source in freshly made cheese, as the initial stages of cheesemaking involve fermentation of lactose to lactate by lactic acid bacteria (Button and Dutton, 2012). We observed a large number of genes (63/2264 or 2.8% of KEGG-annotated genes) involved in lactate import (lactate permease - K03303) or lactate metabolism. Lactate dehydrogenase (K00101), which reduces lactate to pyruvate, represented nearly 1% of all horizontally transferred protein coding sequences.
Apart from lactate, the primary source of energy for microbial growth in cheese is metabolism of the abundant lipids and proteins, particularly casein (Monnet et al., 2015). We also identified glutamate importers (43/2264 or 1.9%, eg. K12942, K10005-K10008) and short peptide/nickel transporters (88/2264 or 3.9% eg. K03305) in our analysis, suggesting pathways for utilization of casein degradation products. Transporters for micronutrients, including phosphonate (K05781, K06163-K06165), molybdate (K02017, K02019, K02020, K03750, K03750, K03639), and metal ions like zinc and manganese were also identified.
HGT of drug resistance genes is of particular concern, as mobile resistance genes from food-associated microbes may be transferred to animal- and human-associated microbes (Rossi et al., 2014). Cheese rind communities often contain filamentous fungi including Penicillium species and other microbes that could potentially produce antimicrobial compounds and thus select for antibiotic resistance in co-occurring species. However, less than 1% of KEGG-annotated genes in this dataset are related to drug resistance. A tetracycline resistance gene was identified in eight Brevibacterium species (group 10) and a tripartite multidrug resistance system (K03446, K03543) in three Pseudomonas species (group 37).
We also noted a small number of genes from the core genome that were not expected to be horizontally transferred. For example, group 27 is found in all 10 strains of B. linens in this dataset, as well as the closely related B. antiquum CNRZ918, and contains the SSU ribosomal protein S1p, as well as DNA Polymerase 1. It is possible that these results are false positives as B. linens and B. antiquum have an ANI ~88%, and these genes are typically more highly conserved than average. At the same time, other ribosomal genes that should also be highly conserved protein coding genes have substantially lower homology between these species than S1p (Supplementary file 1e). Further, another gene within this HGT group (SAM-dependent methyltransferase) is not typically highly conserved, but nevertheless is >99% identical between these Brevibacterium species. We cannot exclude the possibility that this is a false positive, but this may be an example of homologous recombination facilitated by the high sequence identity of the ribosomal protein gene. Several other groups also contain ribosomal proteins (42 L5p and S3p, 180 L4p, 219 S3p), but these groups do not contain any other protein coding genes, and they are clustered with other ribosomal protein coding genes, which is a more typical arrangement.
Iron acquisition HGT
The abundance of iron acquisition genes identified as HGT suggests that iron is a driving force in adaptation to growth on cheese. The largest HGT region we identified in cheese-associated bacteria, Group 1, includes an island of ~47 kbp (~1% of the genome of B. linens JB5) and 34 genes. This island is found in whole or in part in 15 different species in five different Actinobacterial genera (Brachybacterium, Brevibacterium, Corynebacterium, Microbacterium, and Glutamicibacter, formerly Arthrobacter), and one Firmicute (Alkalibacterium). The core of this region, flanked by AraC-like transcriptional regulators (e.g. Ga0099663_102740 and Ga0099663_102753 from JB182), contains several genes predicted to form a siderophore import complex, including two cell-surface associated substrate binding protein genes (Ga0099663_102743–44), two membrane permease genes (Ga0099663_102745–46), and an ATPase subunit (Ga0099663_102747). A siderophore reductase (Ga0099663_102741) is present immediately downstream of the AraC regulator, but has less than 99% identity between the species we analyzed (Figure 3A, Supplementary file 1c). We named this region iRon Uptake/Siderophore Transport Island (RUSTI).10.7554/eLife.22144.006
HGT Groups in Actinobacteria, Firmicutes, and γ-Proteobacteria groups.
(A) The 31 largest HGT groups that contain predominantly Actinobacteria. The areas of circles are scaled to log2(n), where n is the total number of nucleotides in that group for each species. The largest circle size represents the largest HGT group in that phylum. Phylogenies (left) are based on small subunit ribosomal RNA alignment. (B) The 25 largest HGT groups that contain predominantly Firmicutes. (C) The 28 largest groups that contain predominantly γ-Proteobacteria.
DOI:http://dx.doi.org/10.7554/eLife.22144.006
10.7554/eLife.22144.007
Group A: Expected clustering: contiguous genes in multiple species are in a single group.
Although island 6 (i6) lacks one gene present in i1 and i4, (possibly because of a transposon insertion), it is still considered related. Group B: Ambiguous grouping: islands 2 and 3 from species 1 are found on different contigs, but are grouped together. They may be found in close proximity in the genome, but on different sides of a gap in the assembly, or they may be quite distant from each other. The grouping of related genes in species 2 into a single island suggests that they may have been transferred in a single event, but the possibility of two unrelated HGT events landing in the same spot cannot be excluded. Group C: Possible mis-grouping of two HGT events in a single group: although species 4 does not share any genes with species 1 and 2, these islands are nevertheless clustered because of the proximity of coding sequences in species 3. This may correctly represent a single gene cluster that subsequently diverged in each species, or unrelated HGT that happened to insert in close proximity. Group D: Mis-grouping because of mobile element: Mobile elements (outlined in red) found in multiple locations in multiple genomes may insert next to unrelated HGT islands, causing spurious grouping by the algorithm.
DOI:http://dx.doi.org/10.7554/eLife.22144.007
10.7554/eLife.22144.008
Structure of RUSTI islands.
(A) At-scale schematics for genomic context of HGT Group 1 (top) for B. linens JB5 and G. arilaitensis JB182 and alignment of RUSTI operon (bottom). Nucleotide position values (top) refer to contigs Ga0099665_11 and Ga0099663_102 respectively. Dotted line for JB5 depicts regions of the contig that are not shown. Nucleotide position values (bottom) refer to operon starting from stop codon of leading AraC coding sequence. (B) At-scale schematics for genomic context of HGT Group 7 for Halomonas sp. JB37 and V. casei JB196 (top) and alignment of iron and phosphonate metabolism genes (bottom). Nucleotide position values (top) refer to contigs Ga0099667_11 and Ga0099672_104 respectively. Grey lines for JB196 depict gaps in the alignment resulting from insertions in JB37. Nucleotide position values (bottom) refer to operon starting from stop codon of leading protein coding sequence. (C) At-scale schematics for genomic context of HGT Group 31 for S. fleuretti. CIP106114 and S. vitulinus Ma1 (top). For both species, the group is split across two different contigs and nucleotide position values (top) refer to the relative position for that contig. Alignment of iron and phosphonate metabolism genes from Group 31 (bottom).
DOI:http://dx.doi.org/10.7554/eLife.22144.008
Horizontally transferred genes are not always expressed in the recipient genome, because of possible incompatibilities in promoter sequence (Ochman et al., 2000). As iron is a limiting resource in cheese (Kastman et al., 2016; Monnet et al., 2012), we reasoned that if RUSTI is a functional operon, it would likely have increased expression in the presence of additional competition for iron. To assess whether RUSTI genes are regulated in the presence of competition, we grew G. arilaitensis JB182 alone or in the presence of Penicillium and performed RNA sequencing (RNA-seq) to monitor gene expression. The genes in RUSTI were significantly upregulated in the presence of a competing microbe relative to growth alone (Figure 3B, Supplementary file 1f), suggesting that this horizontally transferred region is transcriptionally active and may be responding to competition for limited iron in cheese.
Hundreds of different siderophores have been identified belonging to three major classes: hydroxamate, catecholate, and α-hydroxycarboxylate (Hider and Kong, 2010). To predict the function of the RUSTI transporters, we compared their protein sequences to the Transporter Classification Database (TCDB) (Saier et al., 2016) using BLAST (Supplementary file 1g). The two genes annotated as permease subunits and one gene annotated as an ATP binding subunit each share substantial homology with the catechol ferric enterobactin transport system (FepD, FepG, and FepC, respectively) in E. coli (Elkins and Earhart, 1989; Shea and McIntosh, 1991; Chenault and Earhart, 1991). Two genes annotated as substrate binding proteins have weak homology to vibriobactin and iron(III) dicitrate binding proteins from Vibrio cholerae and E. coli, respectively.
Siderophore-related genes are also well-represented in γ-Proteobacterial HGT groups. Like Group 32 in Actinobacteria, Group 39 contains both siderophore acquisition and siderophore biosynthesis genes and is found in three species of Psychrobacter. The HGT group with the most protein coding genes that we identified in γ-Proteobacteria (Group 7) is found in several Vibrio and Halomonas species, and like ActinoRUSTI contains an ABC siderophore transport system with individual substrate-binding, permease, and ATP-binding domains (Figure 3C). Although this group appears to have analogous function in the acquisition of iron with RUSTI from Actinobacteria, this ProteoRUSTI does not appear to be related. TCDB analysis suggests homology to hemin transporters in Yersinia pestis and Bordetella pertussis (Supplementary file 1g).
The same gene island also contains genes related to the Phn family involved in phosphonate import and metabolism (Jochimsen et al., 2011). Phosphonate metabolism genes have previously been associated with iron siderophore acquisition in acidic environments (Osorio et al., 2008), and cheese is typically close to pH5 during the initial periods of rind community growth. Interestingly, BLAST of this region against the NCBI RefSeq database reveals that several uropathogenic E. coli strains share identical DNA sequences (Supplementary file 1h). Highly similar sequences are found in Oligella urethralis, another gram-negative pathogen of the urogenital tract, and Vibrio harveyi, a bioluminescent ocean-dwelling microbe. Iron sequestration by animals is a common defense against pathogens (Parrow et al., 2013), and enhanced iron acquisition is commonly associated with virulence. Mammals produce lactoferrin in milk for the same reason (Ellison, 1994), and these data suggest that the same genes would be adaptive in both pathogenesis and growth on cheese.
The convergence of strategies for both pathogenesis and growth on cheese was also demonstrated in the Firmicutes (Figure 3D). Two species of cheese-associated Staphylococcus (S. fleuretti CIP106114 and S. vitulinus Ma1) share a large (~20 kb) cluster of genes (Group 31) that includes hemolysin and fibronectin binding protein (FnBP), which are involved in virulence in S. aureus (Sinha et al., 1999; Cheung and Ying, 1994). Hemolysin (also known as alpha toxin) forms pores in cell membranes, and is so-called because of its ability to lyse red blood cells. FnBP enables binding to and invasion of cells, and has been implicated in formation of biofilms in methicillin resistant S. aureus (McCourt et al., 2014). It is unlikely that these genes provide a selective advantage to cheese-associated Staphylococci, but Group 31 also contains genes for iron acquisition. These genes are homologous to the EfeUOB systems in E. coli and Bacillus subtilis and FepABC system in S. aureus, which are active in low-pH conditions (Cao et al., 2007; Miethke et al., 2013; Turlin et al., 2013). These iron acquisition genes are also found in association with hemolysin and FnBP in S. aureus and the animal-associated S. sciuri, although at only ~80% nucleotide identity (Supplementary file 1h) (Kloos and Schleifer, 1976). As iron acquisition genes may be adaptive both on cheese and in pathogenesis, it is possible that this region was acquired from an animal pathogen, and the virulence genes have been preserved because of their association with these genes.
The genomes sequenced are from a limited number of cheeses from Europe and the United States. To determine the distribution of RUSTI across a more expansive sample of cheese microbiomes, we used BLAST searches against assembled metagenomic data from 38 different cheeses using representative Proteo-, Actino-, and Staph-RUSTI sequences (Figure 4). Gene islands at least 97% identical to ActinoRUSTI were readily identified in 23 (61%) of these metagenomes, in both natural and washed rind cheeses from the United States and Europe. Although less common (26% of metagenomes), ProteoRUSTI was also identified in diverse cheeses in both the US and Europe. StaphRUSTI could not be found in any of the metagenomes we analyzed. These data demonstrate that siderophore-associated HGT islands are widespread in cheese rind microbiomes. Whether independent HGT events occur within each cheese production and aging facility, or whether they occurred before the widespread distribution of these microbes across cheese production regions is unknown.10.7554/eLife.22144.009
Presence of RUSTI in cheese metagenomes.
Genes in ActinoRUSTI (G. arilaitensis JB182) and ProteoRUSTI (V. casei JB196) regions were compared with 32 assembled metagenomes from the US and Europe. Filled CDS represents a positive (>97% identical nucleotides) hit in that metagenome.
DOI:http://dx.doi.org/10.7554/eLife.22144.009
A potential mechanism of transfer and source of the Actinobacterial RUSTI
To begin to understand potential mechanisms which could mediate HGT in cheese-associated bacteria, we analyzed the sequences surrounding the RUSTI region of Glutamicibacter JB182. Conjugative elements are commonly involved in HGT (Wozniak and Waldor, 2010). Integrative and conjugative elements (ICEs) can in part be identified by the presence of signature proteins associated with core functions of integration into and excision from the host genome (recombinase), replication as an extrachromosomal element (polymerase), and conjugation from the host to recipient cell (conjugation) (Ghinet et al., 2011). Analysis of the Glutamicibacter JB182 RUSTI region revealed homologs of each of these protein classes (Figure 3A): a recombinase of the site-specific tyrosine recombinase XerD family (Ga0099663_102762) (Subramanya et al., 1997), a hexameric ATPase conjugation protein of the VirD4/TraG/TraD family (Ga0099663_102784) (Hamilton et al., 2000), and a homolog of the bi-functional primase-polymerase DNA replication protein family (Ga0099663_102766). Interestingly, Actinobacterial ICE systems typically use conjugation apparatus belonging to the SpoIIE/FtsK family, which allows transfer of double-stranded DNA (te Poele et al., 2008; Bordeleau et al., 2012). However, the conjugation machinery here is more reminiscent of gram-negative and Firmicute systems of single-stranded transfer (Burrus and Waldor, 2004).
ICE integration is site-specific, and frequently occurs at the 3’ end of tRNA genes (Ghinet et al., 2011). Immediately downstream of the RUSTI region in Glutamicibacter is a leucine tRNA. The 3’ end of the tRNA forms an imperfect repeat with the region immediately upstream of the RUSTI region, which strongly suggests that the tRNA-Leu is used at the integration site (att site) for this ICE. To determine whether this ICE was still active, we performed PCR using primers within and flanking the putative integration site (Figure 5A). We were able to detect PCR products which suggest that at least a portion of the cells within the population have lost the RUSTI ICE from their chromosome, and it is present as an extrachromosomal circular form (Figure 5B). Sequencing of the PCR product (primers 1 + 6) that spans the predicted excision site matched the predicted remaining sequence, containing Repeat element B (Figure 5D). Sequencing of the PCR product (primers 2 + 5) that spans the predicted circularization site matched the predicted sequence, containing Repeat element A (Figure 5E).10.7554/eLife.22144.010
Mobility of RUSTI.
(A) Schematic for PCR primer design - see Materials and methods for details. (B) PCR testing for the presence of RUSTI and for the excision of the ICE in an overnight culture of G. arilaitensis JB182. (C) DNA was extracted from five commercially available starter cultures and tested for the presence of RUSTI using PCR with primers specific for the HGT region (Materials and methods). Starter culture 3 was plated on PCAMS media, and four isolates selected based on colony morphology were also tested. The expected size for the amplicon is ~1.4 kb. Sequencing of the 16S ribosomal RNA genes for these isolates suggested that two isolates are Glutamicibacter arilaitensis and two are Brevibacterium linens. G. arilaitensis. JB182 was used as a positive control. (D) The ~2000 bp band from the PCR amplification using primers 1 and 6 and (E) the ~500 bp band from amplification using primers 2 and 5 were extracted and sequenced. Alignment with the JB182 genome reveals 100% alignment with expected and the spliced chromosomal region containing the 3’ repeat and the excision circle containing the 3’ repeat respectively.
DOI:http://dx.doi.org/10.7554/eLife.22144.010
There are several possible explanations for the widespread distribution of nearly identical ActinoRUSTI. Initial transfer events may have occurred in a single location, on the surface of a cheese or in livestock, and subsequently been dispersed to many separate cheesemaking facilities. The continued mobility of the ICE in JB182 raises the alternate possibility that it may be continually introduced to many cheeses from a common source. Cheese producers often use commercially available ‘starter’ cultures that contain desirable species, including many Actinobacteria (Robinson, 2005). We tested five common starter cultures for the presence of ActinoRUSTI by PCR, and positively identified it in one of these (Figure 5C). This culture is known to contain two species of Actinobacteria, Brevibacterium linens and Glutamicibacter nicotinae. To identify the RUSTI donor species, we plated the starter culture and isolated four distinct strains based on colony morphology. SSU sequencing revealed that two isolates were Glutamicibacter and two were Brevibacterium. One of the Brevibacterium isolates tested positive for ActinoRUSTI by PCR (Figure 5C). Although we have no evidence for direct transmission of ActinoRUSTI from this starter culture, and thus cannot definitively conclude that this was the source, these data are consistent with the hypothesis that HGT from a starter culture could explain some of the dissemination of ActinoRUSTI.
Discussion
In this paper, we provide evidence of extensive horizontal gene transfer in cheese-associated bacteria. Many of the transferred regions are large multi-gene islands, and are shared by numerous species. Genes involved in nutrient acquisition, especially iron and lactate, are particularly abundant, suggesting that HGT may provide a selective advantage in the iron- and sugar-limited environment of cheese. The largest HGT we identified appears to be an active ICE and is found in a starter culture, raising the possibility that we are observing contemporary processes that may have ongoing importance. These data support previous studies that show HGT is an important factor in the evolution of microbial communities (Wiedenbeck and Cohan, 2011) and suggest that cheese rind communities may provide a useful model for studying this process in greater detail.
For this study, we focused on bacterial members of the cheese microbiome, but many cheeses also contain numerous fungal species. Indeed, HGT has been previously documented in cheese-associated Penicillium species (Cheeseman et al., 2014). HGT between bacteria and fungi has also been documented in other environments, although it is thought to be rare (Keeling and Palmer, 2008). Evaluating bacterial-fungal gene transfer in cheese could provide additional insights into the extent and importance of gene exchange in microbial communities. Further sequencing of bacterial genomes from cheese could also continue to reveal HGT.
We were able to show that ActinoRUSTI in G. arilaitensis JB182 is likely contained within an integrative and conjugative element, although many other mechanisms for transfer are likely at play. Indeed, the appearance of phage-related genes, transposases, and other mobile elements in many HGT groups suggests that we are observing the results of multiple methods for mobilizing DNA.
Our method for identifying HGT does not permit determination of the direction of gene flow, and indeed it seems likely that the original sources of many of these genetic elements are not present in our dataset. In some cases, we can infer a possible origin, such as the Brevibacterium strain in a starter culture that may be the source of RUSTI in multiple cheese species around the world. However, this does not permit identification of where this species acquired them. Further characterization of cheese-associated microbes, as well as those found in dairy farms or in cheese caves may provide a more complete picture, but the evidence that at least some of these genetic elements are found in human pathogens and ocean-dwelling bacteria suggests that genes are shared across diverse environments.
Although previous studies demonstrated that iron is limiting for Glutamicibacter, Brevibacterium, and Corynebacterium species growing on cheese (Monnet et al., 2012, 2010), the preponderance of siderophore and other iron acquisition genes we observed being horizontally transferred suggests that the same is true across bacterial phyla. Limiting iron is a deliberate strategy on the part of mammalian hosts to block the growth of infectious microbes, and this strategy influences the composition of milk because of the presence of lactoferrin (Ellison, 1994). Interestingly, convergent strategies for acquiring iron are used by pathogens and by cheese-associated microbes and we observe that in some cases these disparate species appear to have shared genes through horizontal transfer. The presence of these same genes in a microbe found in ocean habitats suggests that these genes have broad utility for the common challenge of iron limitation.
We have yet to demonstrate the functional consequences of these genes on individual species or on the community as a whole. Given that iron is limiting in cheese, and that ActinoRUSTI genes are upregulated in response to other species (Figure 4C), it is likely that these genes are functional and may play a role in competition. The prevalence of siderophore import, but not siderophore synthesis pathways suggests that species may cheat by scavenging the biosynthetic products of others (Cordero et al., 2012).
The identification of widespread sharing of genes in cheese microbial communities could have important implications. In particular, the possibility that a starter culture is the source of mobile gene elements suggests that the genomic content, rather than just specific species, must be considered when designing microbial supplements. In addition to starter cultures used for fermented foods, living microbial supplements (‘probiotics’) are increasingly being adopted in agriculture (Verschuere et al., 2000; Chaucheyras-Durand and Durand, 2010) and for a wide range of human health conditions (Cuello-Garcia et al., 2015; Onubi et al., 2015; (IBS Dietetic Guideline Review Group on behalf of Gastroenterology Specialist Group of the British Dietetic Association) et al., 2016), and even as cosmetics (Whitlock et al., 2016). The need to screen for clinically relevant elements such as antibiotic resistance genes is widely recognized (Sanders et al., 2010), but other mobile gene elements from these organisms may also enter native microbial populations with unknown consequences.
Although we and others have observes a large number of HGT events in microbial species across a diverse range of environments (McDaniel et al., 2010; Smillie et al., 2011; Ravenhall et al., 2015), the biotic and abiotic conditions that affect this frequency, and what effects HGT may have on the community remain unclear. A model system to study HGT in a community context is particularly important, as sequence-based characterization of complex communities has particular limitations when it comes to HGT. Further, even if complete characterization in situ were possible, many microbial communities are difficult to experimentally manipulate in vitro.
By contrast, cheese rind-associated bacteria are readily isolated and cultured, and model communities may enable identification of features of microbial communities and their environments that alter frequency and extent of HGT. The cheese rind model system provides an opportunity to observe HGT as it happens and to investigate how community composition affects the frequency of transfer and the persistence of genes. The in vitro cheese system enables experimental manipulation to investigate the role of community composition in driving HGT. Further, as many gene products may only have survival benefits in the context of community competition and cooperation, investigating the role of RUSTI and other horizontally transferred genes on microbial growth in the context of their natural community is critical. Having an experimentally tractable microbial community will allow us to test these ideas under controlled conditions in the laboratory and generate predictions about how these processes work in more complex natural systems. Many species of cheese-associated bacteria have close relatives in the soil, on skin, in the ocean, making insight from this system potentially applicable to diverse environments. In addition, horizontal acquisition of iron-uptake genes has been documented in numerous environments including the oceans and in human pathogens (Gyles and Boerlin, 2014; Richards et al., 2009), suggesting that the specific processes occurring in cheese may also be generally informative across systems. Understanding the extent of HGT in the cheese microbiome is the first step towards addressing how the movement of genes shapes, and is shaped by, a microbial community. Using cheese rinds as a model system could help elucidate the factors that influence the frequency of HGT, how it impacts competition and cooperation, and helps shape a microbiome.
Materials and methodsSequencing and genome assembly
Bacterial strains JB4, 5, 7, 37, 110, 182, 196, and 197 were isolated from cheeses in a single geographic region and sequenced using a combination of Illumina short-read (100 bp, paired end) and Pacbio long-read sequencing. DNA was extracted using Genomic Tip 100/G (Qiagen, USA) or Power Soil (MoBio, USA). Illumina library preparation and sequencing were performed at Harvard University by the Bauer Core facility. Pacbio library preparation and sequencing were performed by the University of Massachusetts Medical School Deep Sequencing Core. De novo hybrid assembly was performed using SPAdes (v3.5.0) (Bankevich et al., 2012). Genomes were annotated using the Integrated Microbial Genomes Expert Review (IMG/ER) annotation pipeline (Markowitz et al., 2012). In addition, we also sequenced eight additional rind isolates of Brachybacterium (strains 341.9, 738.10, 862.8, 876.9, 900.8, 908.11, 947.1, 962.10) and Brevibacterium (strains 341.13, 738.8, 862.7, 876.7, 900.6, 908.7, 947.7, 962.8) and three additional isolates of Glutamicibacter (strains BW77, 78, 80) from different cheeses in a broad geographic distribution. For these isolates, we prepared draft genomes using Illumina short-read sequencing and assembled with CLC genomics workbench.
The annotated genomes used can be found on Zenodo (Bonham et al., 2016)
Phylogenetic trees
16S sequences were retrieved from the sequenced genomes and aligned using the structure-based aligner, Infernal v1.1rc4 (Nawrocki and Eddy, 2013), as implemented in the Ribosomal Database Project release 11 (Cole et al., 2009). The alignment was imported into Geneious v9 (Biomatters, LTD), and a tree was calculated using the maximum likelihood method PHYML (GTR model)(Guindon et al., 2003). The tree was rooted using Thermus thermophilus. The tree was then uploaded to Interactive Tree of Life (iTOLv3) (Letunic and Bork, 2016) to enable mapping of HGT data (connections and group abundance profiles).
RNAseq
Four replicate transcriptomes from two treatments were sequenced: (1) G. arilaitensis alone and (2) G. arilaitensis + Penicillium. We used a strain of Penicillium solitum that was isolated from a natural rind cheese and was used for experiments in Wolfe et al. (2014). For each experimental unit, approximately 80,000 CFUs of Glutimicibacter arilaitensis were spread across the surface of a 100 mm Petri dish containing 20 mL of cheese curd agar (10% freeze-dried fresh cheese, 3% NaCl, 1.7% agar, 0.5% xanthan gum) (Wolfe et al., 2014). For the + Penicillium treatment, approximately 100,000 CFUs were co-inoculated onto the plates with the Glutimicibacter. Plates were incubated in a bin with moist paper towel (>90% relative humidity) at 24°C for 5 days.
Rind biofilms were then harvested by scraping the cheese curd surface and stored in RNAProtect Reagent (Qiagen) to stabilize mRNA frozen at −80°C. RNA was extracted using a standard phenol-chloroform protocol used for many different fungal and bacterial species, which has been adopted from transcriptomics work in gut microbiomes (see [David et al., 2014]). This protocol uses a standard bead-beating step in a lysis buffer to release cell contents from biofilms stored in RNAProtect. DNA was removed from the samples using a TURBO DNA-free kit (Life Technologies), and 5S tRNA and large rRNA was depleted using MEGAClear (Life Technologies) and RiboZero (Illumina) kits, respectively. To remove both fungal and bacterial large rRNA, we used an equal mixture of Ribo-Zero Yeast and Bacteria rRNA Removal Solution. To confirm that the samples were free of DNA contaminants, a PCR of the 16S rRNA gene was performed with standard primers (27 f and 1492 r). Overall quantity and quality of the RNA preps were confirmed by Nanodrop and Agilent 2100 Bioanalyzer using the RNA 6000 Nano kit.
RNA-seq libraries were constructed from purified mRNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) where each library received a unique six base pair barcode for demultiplexing after the sequencing run. Each library was run on an Agilent 2100 Bioanalyzer High Sensitivity DNA chip to confirm that primer dimers and adapter dimers were not present in the sample and to determine the size of the library. Final libraries were standardized to 10 nM each after quantification with a Qubit dsDNA HS Assay Kit (Life Technologies) and then pooled in equal amounts to get similar sequencing coverage across all libraries. The pooled library samples were sequenced using paired-end 100 bp reads on an Illumina HiSeq Rapid Run by the Harvard Bauer Core Sequencing Core Facility.
To quantify gene expression and determine whether genes within RUSTI were differentially expressed when grown with the competitor Penicillium, we used Rockhopper (McClure et al., 2013). Only forward reads were used for this analysis. The assembled and annotated Glutamicibacter arilaitensis strain JB182 (described above) genome were used as a reference genome for mapping. We considered genes that had a greater than fourfold difference in expression when grown with Penicillium or Staphylococcus and were significantly different (based on Rockhopper’s q-values, which control for false discovery rate using the Benjamini-Hochberg procedure) to be differentially expressed genes.
PCR
PCR reactions were performed using Q5 Hot Start Mastermix (New England Biolabs). Where JB182 RUSTI is integrated in the chromosome, PCR using primer 1 (CAACTGTGCCACGCAATTCA) and primer 2 (CGGCTACTTCTCGGATGGTC) are expected to produce a 1037 bp product that includes the 5’ ICE repeat. Primer 3 (CGCAATCGTGGTGTATCTGC) and primer 4 (GACGGGATCAGGAACGACG) should produce a 1410 bp product, while primer 5 (GCCGCATCTACCTCGATGAA) and primer 6 (CCAAATCGCGACGCATTGAT) are expected to form a 1467 bp product. Primers 1 and 6 are separated by approximately 59 kb when RUSTI is present and are not expected to form a PCR product, but should form a 1937 bp product if RUSTI is excised. Primers 2 and 5 should not form a PCR product when RUSTI is integrated, but would form a 500 bp product if the excision circle were present.
Additional software
Annotated genomes were compared using blastn from BLAST+ (v2.3.0) (Camacho et al., 2009). Protein coding genes were considered to be potential HGT if their sequence was at least 99% identical for at least 500 nucleotides. Neighboring candidate HGT were identified as part of the same island if they were separated by no more than 5000 nucleotides. Scripts to import and store genome information and blast results and to analyze results are available on github (Bonham, 2016; https://github.com/kescobo/kvasir). A copy is archived at https://github.com/elifesciences-publications/kvasir.
Genomic average nucleotide identity (ANI) was calculated using the ‘ani.rb’ script from the enveomics collection (commit ‘e8faed01ff848222afb5820595cccc4e50c89992’) with default settings (Rodriguez-R and Konstantinidis, 2016).
Metagenomes
Shotgun metagenomic data from (Wolfe et al., 2014) and (Kastman et al., 2016) were assembled with CLC Genomic Workbench 8.0. Representative sequences for ActinoRUSTI or ProteoRUSTI were compared with assembled metagenomes by BLAST. Hits with >97% similarity were considered to be positive hits for target regions.
Accession numbers
Newly sequenced genomes were registered with NCBI with the bioproject ID PRJNA387187. Biosample accession numbers for individual genomes are shown in Supplementary file 1a, and are as follows:
The authors would like to thank Rajashree Mishra for assisting with initial stages of project development, Dana Boyd for assistance with ICE identification, Brian Tsu for assistance with the Transporter Classification Database, and members of the Dutton lab for helpful conversations.
Additional informationCompeting interests
The authors declare that no competing interests exist.
Author contributions
KSB, Conceptualization, Investigation, Writing—original draft, Writing—review and editing
BEW, Conceptualization, Investigation, Writing—review and editing
RJD, Conceptualization, Investigation, Writing—original draft, Writing—review and editing
Additional files10.7554/eLife.22144.011
Genome information.
(a) Genome statistics for newly sequenced genomes, determined by IMG/ER. Gene IDs refer to IMG bioproject or RefSeq Accession. Genomes from Almeida et. al. do not yet have accession numbers. (b) Pairwise species comparison summary total. Protein coding sequences (column ‘Shared CDS’) and nucleotides (in base-pairs - column ‘Shared nt’) determined to be horizontally transferred for every pair of species that were compared by the HGT detection pipeline. Also shows calculated ANI and 16S similarity in % (column ssu - see Materials and methods for method of determining 16S similarity). Species pairs that have ANI > 0.89 were not compared and are not shown. (c) HGT identification parameters. Different parameters for minimum length of gene match for HGT, maximum % ANI identity for related species, and maximum distance between genes in an island were compared. Number of positive HGT hits identified when varying the minimum protein coding gene length. Number of HGT groups constructed when varying the maximum separation between hits that are classified as belonging to the same group. Number of nucleotides or number of protein coding sequences in HGT regions by 16S similarity. Note - There are no results below 500 as 500 bp is the minimum length for protein coding sequences in this analysis. (d) Full group annotations. All protein coding sequences identified as HGT, sorted by group # (ranked by total nucleotide content), species and genome location within species. Certain functional annotations are identified by color (e.g. orange for iron) based on text in annotation. Locus tags and contig IDs beginning with lower case letters were assigned by kvasir, and do not correspond to any published database. (e) Group summary statistics. Summary statistics for each HGT group. (f) Highly conserved genes in Brevibacterium species. Protein coding sequences from Group 29, as well as selected highly conserved genes from Brevibacterium antiquum CNRZ918 were compared with other Brevibacterium strains by BLAST. B. linens 947.7 has substantially lower nucleotide identity for the four genes found in Group 29 than other B. linens strains, despite similar nt distance for other highly conserved genes. This suggests that Group 29 is a true example of HGT between CNRZ918 and other B. linens strains, rather than a false positive. (g) RUSTI gene expression during competition. Gene expression data from RNA seq analysis for genes in JB182 RUSTI. Related to Figure 3B (h) TCBD hits for transporters in RUSTI. Representative CDS of Actino- and ProteoRUSTI from G. arilaitensis JB182 and V. casei JB196, respectively, were compared with the Transporter Classification Database (TCDB). (i) RefSeq BLAST Actino- and ProteoRUSTI from G. arilaitensis JB182 and V. casei JB196, respectively, as well as the consensus sequence for StaphRUSTI (see Figures 3 and 4) were compared with the NCBI RefSeq database using BLAST.
DOI:http://dx.doi.org/10.7554/eLife.22144.011
Major datasets
The following datasets were generated:
Bonham KS,Wolfe BE,Dutton RJ,2016,Datasets associated with Bonham et al.,https://doi.org/10.5281/zenodo.163212,Publicly available at Zenodo (https://zenodo.org)
Bonham KS,Wolfe BE,Dutton RJ,2017,Datasets associated with Bonham et al.,http://www.ncbi.nlm.nih.gov/bioproject/387187,Publicly available at the NCBI BioProject (accession no: PRJNA387187)
The following previously published datasets were used:
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524487.3,Publicly available at MG-RAST (accession no: 4524487.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524500.3,Publicly available at MG-RAST (accession no: 4524500.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524498.3,Publicly available at MG-RAST (accession no: 4524498.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524496.3,Publicly available at MG-RAST (accession no: 4524496.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524502.3,Publicly available at MG-RAST (accession no: 4524502.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524495.3,Publicly available at MG-RAST (accession no: 4524495.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524488.3,Publicly available at MG-RAST (accession no: 4524488.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524490.3,Publicly available at MG-RAST (accession no: 4524490.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524499.3,Publicly available at MG-RAST (accession no: 4524499.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524497.3,Publicly available at MG-RAST (accession no: 4524497.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524491.3,Publicly available at MG-RAST (accession no: 4524491.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524493.3,Publicly available at MG-RAST (accession no: 4524493.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524501.3,Publicly available at MG-RAST (accession no: 4524501.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524482.3,Publicly available at MG-RAST (accession no: 4524482.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524489.3,Publicly available at MG-RAST (accession no: 4524489.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524483.3,Publicly available at MG-RAST (accession no: 4524483.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524505.3,Publicly available at MG-RAST (accession no: 4524505.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524494.3,Publicly available at MG-RAST (accession no: 4524494.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524486.3,Publicly available at MG-RAST (accession no: 4524486.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524504.3,Publicly available at MG-RAST (accession no: 4524504.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524485.3,Publicly available at MG-RAST (accession no: 4524485.3)
Wolfe BE,Dutton RJ,2014,shotgun metagenomic data from cheese rinds used in Figure 4,http://metagenomics.anl.gov/mgmain.html?mgpage=overview&metagenome=mgm4524484.3,Publicly available at MG-RAST (accession no: 4524484.3)
ReferencesAlmeidaMHébertAAbrahamALRasmussenSMonnetCPonsNDelbèsCLouxVBattoJMLeonardPKennedySEhrlichSDPopMMontelMCIrlingerFRenaultP2014Construction of a dairy microbial genome catalog opens new perspectives for the metagenomic analysis of dairy fermented productsBMC Genomics15110110.1186/1471-2164-15-110125496341AndamCPCarverSMBerthrongST2015Horizontal gene flow in Managed EcosystemsAnnual Review of Ecology, Evolution, and Systematics4612114310.1146/annurev-ecolsys-112414-054126BankevichANurkSAntipovDGurevichAADvorkinMKulikovASLesinVMNikolenkoSIPhamSPrjibelskiADPyshkinAVSirotkinAVVyahhiNTeslerGAlekseyevMAPevznerPA2012SPAdes: a new genome assembly algorithm and its applications to single-cell sequencingJournal of Computational Biology1945547710.1089/cmb.2012.002122506599BonhamKSWolfeBEDuttonRJ2016Datasets associated with Bonham, et alZenodoBonhamKS2016Kvasir: release for publicationGithub43fb8ae768b174114b227aa0c0639eaa73bcc0b6https://github.com/kescobo/kvasirBordeleauEGhinetMGBurrusV2012Diversity of integrating conjugative elements in Actinobacteria: coexistence of two mechanistically different DNA-translocation systemsMobile Genetic Elements211912410.4161/mge.2049822934248BurrusVWaldorMK2004Shaping bacterial genomes with integrative and conjugative elementsResearch in Microbiology15537638610.1016/j.resmic.2004.01.01215207870ButtonJEDuttonRJ2012Cheese microbesCurrent Biology22R587R58910.1016/j.cub.2012.06.01422877773CamachoCCoulourisGAvagyanVMaNPapadopoulosJBealerKMaddenTL2009BLAST+: architecture and applicationsBMC Bioinformatics1042110.1186/1471-2105-10-42120003500CaoJWoodhallMRAlvarezJCartronMLAndrewsSC2007EfeUOB (YcdNOB) is a tripartite, acid-induced and CpxAR-regulated, low-pH Fe2+ transporter that is cryptic in Escherichia coli K-12 but functional in E. coli O157:H7Molecular Microbiology6585787510.1111/j.1365-2958.2007.05802.x17627767ChanJZHalachevMRLomanNJConstantinidouCPallenMJ2012Defining bacterial species in the genomic era: insights from the genus AcinetobacterBMC Microbiology1230210.1186/1471-2180-12-30223259572Chaucheyras-DurandFDurandH2010Probiotics in animal nutrition and healthBeneficial Microbes13910.3920/BM2008.100221840795CheesemanKRoparsJRenaultPDupontJGouzyJBrancaAAbrahamALCeppiMConseillerEDebuchyRMalagnacFGoarinASilarPLacosteSSalletEBensimonAGiraudTBrygooY2014Multiple recent horizontal transfers of a large genomic region in cheese making fungiNature Communications5287610.1038/ncomms387624407037ChenaultSSEarhartCF1991Organization of genes encoding membrane proteins of the Escherichia coli ferrienterobactin permeaseMolecular Microbiology51405141310.1111/j.1365-2958.1991.tb00787.x1787794CheungALYingP1994Regulation of alpha- and beta-hemolysins by the sar locus of Staphylococcus aureusJournal of Bacteriology17658058510.1128/jb.176.3.580-585.19947507919CocconcelliPSCattivelliDGazzolaS2003Gene transfer of vancomycin and tetracycline resistances among Enterococcus faecalis during cheese and sausage fermentationsInternational Journal of Food Microbiology8831532310.1016/S0168-1605(03)00194-614597004ColeJRWangQCardenasEFishJChaiBFarrisRJKulam-Syed-MohideenASMcGarrellDMMarshTGarrityGMTiedjeJM2009The Ribosomal Database Project: improved alignments and new tools for rRNA analysisNucleic Acids Research37D141D14510.1093/nar/gkn87919004872CoombsJMBarkayT2004Molecular evidence for the evolution of metal homeostasis genes by lateral gene transfer in Bacteria from the deep terrestrial subsurfaceApplied and Environmental Microbiology701698170710.1128/AEM.70.3.1698-1707.200415006795CorderoOXVentourasLADeLongEFPolzMF2012Public good dynamics drive evolution of iron acquisition strategies in natural bacterioplankton populationsPNAS109200592006410.1073/pnas.121334410923169633Cuello-GarciaCABro?ekJLFiocchiAPawankarRYepes-Nu?ezJuan Jos?TerraccianoLGandhiSAgarwalAZhangYSch?nemannHJ2015Probiotics for the prevention of allergy: a systematic review and meta-analysis of randomized controlled trialsJournal of Allergy and Clinical Immunology13695296110.1016/j.jaci.2015.04.031DavidLAMauriceCFCarmodyRNGootenbergDBButtonJEWolfeBELingAVDevlinASVarmaYFischbachMABiddingerSBDuttonRJTurnbaughPJ2014Diet rapidly and reproducibly alters the human gut microbiomeNature50555956310.1038/nature1282024336217DelormeC2008Safety assessment of dairy microorganisms: streptococcus thermophilusInternational Journal of Food Microbiology12627427710.1016/j.ijfoodmicro.2007.08.01417822794ElkinsMFEarhartCF1989Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepBJournal of Bacteriology1715443545110.1128/jb.171.10.5443-5451.19892529253EllisonRT1994The effects of lactoferrin on gram-negative bacteriaAdvances in Experimental Medicine and Biology357719010.1007/978-1-4615-2548-6_87762448FlórezABDelgadoSMayoB2005Antimicrobial susceptibility of lactic acid Bacteria isolated from a cheese environmentCanadian Journal of Microbiology51515810.1139/w04-11415782234ForsbergKJReyesAWangBSelleckEMSommerMODantasG2012The shared antibiotic resistome of soil Bacteria and human pathogensScience3371107111110.1126/science.122076122936781FrischerMEStewartGJPaulJH1994Plasmid transfer to indigenous marine bacterial populations by natural transformationFEMS Microbiology Ecology1512713510.1111/j.1574-6941.1994.tb00237.xGhinetMGBordeleauEBeaudinJBrzezinskiRRoySBurrusV2011Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteriaPLoS One6e2784610.1371/journal.pone.002784622114709GibbonsJGRinkerDC2015The genomics of microbial domestication in the fermented food environmentCurrent Opinion in Genetics & Development351810.1016/j.gde.2015.07.00326338497GuindonSGascuelOA simpleGO2003A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihoodSystematic Biology5269670410.1080/1063515039023552014530136GylesCBoerlinP2014Horizontally transferred genetic elements and their role in pathogenesis of bacterial diseaseVeterinary Pathology5132834010.1177/030098581351113124318976HamiltonCMLeeHLiPLCookDMPiperKRvon BodmanSBLankaEReamWFarrandSK2000TraG from RP4 and TraG and VirD4 from Ti plasmids confer relaxosome specificity to the conjugal transfer system of pTiC58Journal of Bacteriology1821541154810.1128/JB.182.6.1541-1548.200010692358HehemannJHCorrecGBarbeyronTHelbertWCzjzekMMichelG2010Transfer of carbohydrate-active enzymes from marine Bacteria to japanese gut MicrobiotaNature46490891210.1038/nature0893720376150HeuerHSmallaK2012Plasmids foster diversification and adaptation of bacterial populations in soilFEMS Microbiology Reviews361083110410.1111/j.1574-6976.2012.00337.x22393901HiderRCKongX2010Chemistry and biology of siderophoresNatural Product Reports2763710.1039/b906679a20376388HiramatsuKCuiLKurodaMItoT2001The emergence and evolution of methicillin-resistant Staphylococcus aureusTrends in Microbiology948649310.1016/S0966-842X(01)02175-811597450JochimsenBLolleSMcSorleyFRNabiMStougaardJZechelDLHove-JensenB2011Five phosphonate operon gene products as components of a multi-subunit complex of the carbon-phosphorus lyase pathwayPNAS108113931139810.1073/pnas.110492210821705661KanehisaMSatoYMorishimaKBlastKOALAMK2016BlastKOALA and GhostKOALA: kegg Tools for functional characterization of Genome and Metagenome sequencesJournal of Molecular Biology42872673110.1016/j.jmb.2015.11.00626585406KastmanEKKamelamelaNNorvilleJWCosettaCMDuttonRJWolfeBE2016Biotic interactions shape the Ecological Distributions of Staphylococcus speciesmBio7e01157-1610.1128/mBio.01157-1627795388KeelingPJPalmerJD2008Horizontal gene transfer in eukaryotic evolutionNature Reviews Genetics960561810.1038/nrg238618591983KloosWESchleiferKH1976Characterization of Staphylococcus sciuri sp. nov. and its Subspecies1International Journal of Systematic and Evolutionary Microbiology26223710.1099/00207713-26-1-22LetunicIBorkP2016Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other treesNucleic Acids Research44W242W24510.1093/nar/gkw29027095192LiXXingJLiBYuFLanXLiuJ2013Phylogenetic analysis reveals the coexistence of interfamily and interspecies horizontal gene transfer in Streptococcus thermophilus strains isolated from the same yoghurtMolecular Phylogenetics and Evolution6928629210.1016/j.ympev.2013.06.00223769954LiuMSiezenRJNautaA2009In silico prediction of horizontal gene transfer events in Lactobacillus bulgaricus and Streptococcus thermophilus reveals protocooperation in yogurt manufacturingApplied and Environmental Microbiology754120412910.1128/AEM.02898-0819395564MarkowitzVMChenIMPalaniappanKChuKSzetoEGrechkinYRatnerAJacobBHuangJWilliamsPHuntemannMAndersonIMavromatisKIvanovaNNKyrpidesNC2012IMG: the Integrated Microbial Genomes database and comparative analysis systemNucleic Acids Research40D115D12210.1093/nar/gkr104422194640MathurSSinghR2005Antibiotic resistance in food lactic acid Bacteria--a reviewInternational Journal of Food Microbiology10528129510.1016/j.ijfoodmicro.2005.03.00816289406McCarthyAJLoefflerAWitneyAAGouldKALloydDHLindsayJA2014Extensive horizontal gene transfer during Staphylococcus aureus co-colonization in vivoGenome Biology and Evolution62697270810.1093/gbe/evu21425260585McClureRBalasubramanianDSunYBobrovskyyMSumbyPGencoCAVanderpoolCKTjadenB2013Computational analysis of bacterial RNA-Seq dataNucleic Acids Research41e14010.1093/nar/gkt44423716638McCourtJO'HalloranDPMcCarthyHO'GaraJPGeogheganJA2014Fibronectin-binding proteins are required for biofilm formation by community-associated methicillin-resistant Staphylococcus aureus strain LACFEMS Microbiology Letters35315716410.1111/1574-6968.1242424628034McDanielLDYoungEDelaneyJRuhnauFRitchieKBPaulJH2010High frequency of horizontal gene transfer in the oceansScience3305010.1126/science.119224320929803(IBS Dietetic Guideline Review Group on behalf of Gastroenterology Specialist Group of the British Dietetic Association)McKenzieYAThompsonJGuliaPLomerMC2016British Dietetic Association systematic review of systematic reviews and evidence-based practice guidelines for the use of probiotics in the management of irritable bowel syndrome in adults (2016 update)Journal of Human Nutrition and Dietetics2957659210.1111/jhn.1238627265510MiethkeMMonteferranteCGMarahielMAvan DijlJM2013The Bacillus subtilis EfeUOB transporter is essential for high-affinity acquisition of ferrous and ferric ironBiochimica Et Biophysica Acta18332267227810.1016/j.bbamcr.2013.05.02723764491MonnetCBackAIrlingerF2012Growth of aerobic ripening Bacteria at the cheese surface is limited by the availability of ironApplied and Environmental Microbiology783185319210.1128/AEM.00085-1222367081MonnetCLandaudSBonnarmePSwennenD2015Growth and adaptation of microorganisms on the cheese surfaceFEMS Microbiology Letters3621910.1093/femsle/fnu02525790503MonnetCLouxVGibratJFSpinnlerEBarbeVVacherieBGavoryFGourbeyreESiguierPChandlerMElleuchRIrlingerFVallaeysT2010The Arthrobacter arilaitensis Re117 genome sequence reveals its genetic adaptation to the surface of cheesePLoS One5e1548910.1371/journal.pone.001548921124797NawrockiEPEddySR2013Infernal 1.1: 100-fold faster RNA homology searchesBioinformatics292933293510.1093/bioinformatics/btt50924008419NemergutDRSchmidtSKFukamiTO'NeillSPBilinskiTMStanishLFKnelmanJEDarcyJLLynchRCWickeyPFerrenbergS2013Patterns and processes of microbial community assemblyMicrobiology and Molecular Biology Reviews7734235610.1128/MMBR.00051-1224006468NiehusRMitriSFletcherAGFosterKR2015Migration and horizontal gene transfer divide microbial genomes into multiple nichesNature Communications6892410.1038/ncomms992426592443OchmanHLawrenceJGGroismanEA2000Lateral gene transfer and the nature of bacterial innovationNature40529930410.1038/3501250010830951OnubiOJPoobalanASDineenBMaraisDMcNeillG2015Effects of probiotics on child growth: a systematic reviewJournal of Health, Population and Nutrition34810.1186/s41043-015-0010-426825706OsorioHMartínezVNietoPAHolmesDSQuatriniR2008Microbial iron management mechanisms in extremely acidic environments: comparative genomics evidence for diversity and versatilityBMC Microbiology820310.1186/1471-2180-8-20319025650ParrowNLFlemingREMinnickMF2013Sequestration and scavenging of iron in infectionInfection and Immunity813503351410.1128/IAI.00602-1323836822RavenhallMŠkuncaNLassalleFDessimozC2015Inferring horizontal gene transferPLOS Computational Biology11e100409510.1371/journal.pcbi.100409526020646RichardsTASoanesDMFosterPGLeonardGThorntonCRTalbotNJ2009Phylogenomic analysis demonstrates a pattern of rare and ancient horizontal gene transfer between plants and fungiThe Plant Cell Online211897191110.1105/tpc.109.06580519584142RobinsonRK2005Dairy Microbiology Handbook: The Microbiology of Milk and Milk ProductsWileyRodriguez-RLMKonstantinidisKT2016The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomesPeerJ Preprintse1900v1RoparsJRodríguez de la VegaRCLópez-VillavicencioMGouzyJSalletEDumasÉLacosteSDebuchyRDupontJBrancaAGiraudT2015Adaptive horizontal gene transfers between multiple Cheese-Associated fungiCurrent Biology252562256910.1016/j.cub.2015.08.02526412136RossiFRizzottiLFelisGETorrianiS2014Horizontal gene transfer among microorganisms in food: current knowledge and future perspectivesFood Microbiology4223224310.1016/j.fm.2014.04.00424929742SaierMHReddyVSTsuBVAhmedMSLiCMoreno-HagelsiebG2016The Transporter classification database (TCDB): recent advancesNucleic Acids Research44D372D37910.1093/nar/gkv110326546518SandersMEAkkermansLMHallerDHammermanCHeimbachJHörmannspergerGHuysGLevyDDLutgendorffFMackDPhothirathPSolano-AguilarGVaughanE2010Safety assessment of probiotics for human useGut Microbes116418510.4161/gmic.1.3.1212721327023SheaCMMcIntoshMA1991Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coliMolecular Microbiology51415142810.1111/j.1365-2958.1991.tb00788.x1838574SinhaBFrançoisPPNüsseOFotiMHartfordOMVaudauxPFosterTJLewDPHerrmannMKrauseKH1999Fibronectin-binding protein acts as Staphylococcus aureus invasin via fibronectin bridging to integrin alpha5beta1Cellular Microbiology110111710.1046/j.1462-5822.1999.00011.x11207545SmillieCSSmithMBFriedmanJCorderoOXDavidLAAlmEJ2011Ecology drives a global network of gene exchange connecting the human microbiomeNature48024124410.1038/nature1057122037308SubramanyaHSArciszewskaLKBakerRABirdLESherrattDJWigleyDB1997Crystal structure of the site-specific recombinase, XerDThe EMBO Journal165178518710.1093/emboj/16.17.51789311978TasseLBercoviciJPizzut-SerinSRobePTapJKloppCCantarelBLCoutinhoPMHenrissatBLeclercMDoréJMonsanPRemaud-SimeonMPotocki-VeroneseG2010Functional metagenomics to mine the human gut microbiome for dietary fiber catabolic enzymesGenome Research201605161210.1101/gr.108332.11020841432te PoeleEMBolhuisHDijkhuizenL2008Actinomycete integrative and conjugative elementsAntonie Van Leeuwenhoek9412714310.1007/s10482-008-9255-x18523858TurlinEDébarbouilléMAugustyniakKGillesAMWandersmanC2013Staphylococcus aureus FepA and FepB proteins drive heme iron utilization in Escherichia coliPLoS One8e5652910.1371/journal.pone.005652923437157VerschuereLRombautGSorgeloosPVerstraeteW2000Probiotic Bacteria as biological control agents in aquacultureMicrobiology and Molecular Biology Reviews6465567110.1128/MMBR.64.4.655-671.200011104813WalterFAlbersmeierAKalinowskiJRückertC2014Complete genome sequence of Corynebacterium casei LMG S-19264T (=DSM 44701T), isolated from a smear-ripened cheeseJournal of Biotechnology189767710.1016/j.jbiotec.2014.08.03825193709WangHHManuzonMLehmanMWanKLuoHWittumTEYousefABakaletzLO2006Food commensal microbes as a potentially important avenue in transmitting antibiotic resistance genesFEMS Microbiology Letters25422623110.1111/j.1574-6968.2005.00030.x16445749WhitlockDRJamasSWeissL2016Ammonia oxidizing Bacteria for treatment of acneUS PatentUS20160151427https://www.google.com/patents/US20160151427WidderSAllenRJPfeifferTCurtisTPWiufCSloanWTCorderoOXBrownSPMomeniBShouWKettleHFlintHJHaasAFLarocheBKreftJURaineyPBFreilichSSchusterSMilferstedtKvan der MeerJRGroβkopfTHuismanJFreeAPicioreanuCQuinceCKlapperILabartheSSmetsBFWangHSoyerOSIsaac Newton Institute Fellows2016Challenges in microbial ecology: building predictive understanding of community function and dynamicsThe ISME Journal102557256810.1038/ismej.2016.4527022995WiedenbeckJCohanFM2011Origins of bacterial diversity through horizontal genetic transfer and adaptation to new ecological nichesFEMS Microbiology Reviews3595797610.1111/j.1574-6976.2011.00292.x21711367WolfeBEButtonJESantarelliMDuttonRJ2014Cheese rind communities provide tractable systems for in situ and in vitro studies of microbial diversityCell15842243310.1016/j.cell.2014.05.04125036636WozniakRAWaldorMK2010Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flowNature Reviews Microbiology855256310.1038/nrmicro23822060196510.7554/eLife.22144.060Decision letterHandleyKimReviewing editorThe University of Auckland, New Zealand
In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.
Thank you for submitting your article "Extensive Horizontal Gene Transfer in Cheese-Associated Bacteria" for consideration by eLife. Your article has been favorably evaluated by Wendy Garrett (Senior Editor) and three reviewers, one of whom, Kim Handley (Reviewer #1), served as Guest Reviewing Editor. The following individuals involved in review of your submission have agreed to reveal their identity: Danilo Ercolini (Reviewer #2) and Jennifer Martiny (Reviewer #3).
The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.
Summary:
The reviewers agree that this is an interesting and timely study on bacterial HGT using cheese rinds as a model system. The reviewers also raised some important points that should be addressed in a revised version as summarized below.
Essential revisions:
Please provide further evidence to support the conclusions that the gene groups identified do in fact represent HGT as commented on by reviewers 1 and 3. In order to keep the conclusion that HGT is prevalent in cheese rind communities it is also necessary to provide a frame of reference or control group, for example how does this compare in other systems? Reviewer 2 also has made some reasonable requests to add some caveats or temper (tone down) statements in places, e.g. regarding the starting culture as a source of HGT, although we do agree this is an interesting and convincing finding.
Reviewer #1:
The study uses cheese rinds as model environments in which to study microbial HGT. It identifies HGT among a large number of geographically diverse cheese rind microorganisms, and shows that many acquired islands contain genes important for Fe acquisition in an Fe limited environment. The authors demonstrate a prevalent HGT island is actively mobile, and is also present in a commonly used starter culture indicating a possible mechanism for global distribution. This is a well thought out and thorough study that advances our understanding of HGT in microbial communities.
A major conclusion of the study is that HGT is high in cheese rind communities. However, there is no point of reference on which to base this conclusion. High compared to what?
The authors identified mobile elements and insertion sites indicative of genomic islands for one HGT group (RUSTI). It would be useful to know (and strengthen the study) what other HGT groups identified are associated with mobile elements, and what proportion of the total groups. Also Given bacterial genomes can contain a number of putative genomic islands, what is the proportion identified HGT groups to genomic islands not horizontally transferred among the organisms compared?
Reviewer #2:
The topic is timely, the results are very interesting and adequately discussed.
HGT is "the" fascinating source of microbial diversity and here we can see how such diversity can be potentially spread in the complex communities of cheese rinds and how it could play an important role in adaptation to the environment.
I have the following considerations and a few editing suggestions I would expect the authors will consider in revising their manuscript.
“During the aging of cheese, bacteria and fungi form a multi-species biofilm called the rind [Button and Dutton, 2012]”. Such a statement must be changed. This does not apply to just any cheese. So please amend indicating in what types of cheese this happens.
Subsection “Iron Acquisition HGT”, sixth paragraph. This is unlikely to play a role in adaptation, at least in the case of cheese. In addition, the authors do not discuss the Staph HTG. I would just leave out this part. Overall it stands as an awkward deviation in a nice story.
Subsection “A potential mechanism of transfer and source of the Actinobacterial RUSTI”, last paragraph. Although this is a convincing initial indication, it should be proven in a remarkably higher number of cases. I recommend the authors to down-tone the statements in this paragraph to make the conclusions more careful and scaled to the only experiment and case reported.
Discussion, seventh paragraph. This is far too alarming for the readership. HTG may just happily take place naturally in any ecosystem. I suggest rethinking this bit of Discussion.
Discussion, ninth paragraph. Honestly I do not believe in in vitro models. I know the authors already have experience with this. My opinion is that what happens in reconstituted communities with no cheese around can be very different from what could take place in a real system. I would expect the authors to at least recognize the limitations of in vitro studies of food communities in this part of the Discussion.
Reviewer #3:
This article investigates evidence for horizontal gene transfer among bacterial genomes associated with cheese. The manuscript is well-written, the figures are clear, and the conclusions are well-supported. It is not surprising that HGT is prevalent among these bacteria, but it is useful to consider what types of genes are involved to develop cheese as a model system.
I don't have major substantive comments, but did wonder whether the authors had considered doing any analysis of the genomic location of the HGT groups, at least for the large groups. If similar groups are found in very different genomic locations, even among closely related strains, then this would provide evidence for HGT. For instance, this type of analysis would be useful for group 27 and its presence among the B. linens strains (subsection “Functions encoded in HGT regions”, sixth paragraph). Our group has been trying to consider these patterns for HGT in bacteriophage (http://www.sciencedirect.com/science/article/pii/S0042682216302756), and perhaps some consideration of this might help to support or refute the alternatives proposed. However, this is not essential for publication.
10.7554/eLife.22144.061Author response
Essential revisions:
Please provide further evidence to support the conclusions that the gene groups identified do in fact represent HGT as commented on by reviewers 1 and 3.
In order to support the conclusion that the groups identified in the manuscript represent HGT, reviewer 1 requests that we report the extent to which our HGT groups contain genes associated with mobile elements. In our analysis of gene functions encoded by the HGT regions, we found that gene functions associated with mobile genetic elements are the most abundant function identified:
“the most abundant gene functions are transposases, conjugal transfer genes, phage-related proteins and other mobile elements (631/4733 or 14% of all protein coding sequences). Nearly a third (86/264 or 32.6%) of all HGT groups contain mobile elements. These genetic elements are likely involved in either directly or indirectly in the mobilization and transfer of DNA.”
We have now added an additional column in Supplementary file 1E to indicate which of the HGT groups identified in our study contain genes associated with mobile elements and an additional column to Supplementary file 1A which indicates the number of HGT groups found in each genome that we sequenced. Unfortunately, since we are looking only at recent transfer events (by filtering on genes that are >99% identical), and since our method would not identify islands that are horizontally acquired from an organism that we have not sequenced, we are certainly undercounting HGT in these genomes, and there are likely many more genomic islands present. Reviewer 3 suggested analyzing the genomic location of the HGT groups, with the idea that different locations in different genomes would support HGT. While we think this could be interesting to analyze, the presence of an HGT group at the same genomic location in multiple genomes does not rule out HGT. Certain mobile elements, such as integrative and conjugative elements, are known to integrate at the same genomic site due to conserved sequences (such as tRNAs). Also, HGT that occurs via transformation and homologous recombination should not lead to changes in the genomic location of groups.
In order to keep the conclusion that HGT is prevalent in cheese rind communities it is also necessary to provide a frame of reference or control group, for example how does this compare in other systems?
In this study, we identified several hundred potential horizontally transferred regions mapping to two or more genomes amongst the 165 genomes we analyzed. In some cases, these regions were found in numerous genomes (33 genomes in two cases). For this reason, we referred to HGT as being “extensive” in this dataset. However, as the reviewers point out, it is extremely helpful to put this into context of other environments.
Indeed, previous studies indicate that HGT can occur very frequently in natural ecosystems such as the ocean, the human microbiome, and in particular within biofilms (and we have added additional references to the text to highlight this point). As a result, it was not our intention to claim that there is more HGT in cheese than in other environments, merely that we find many examples of HGT in cheese-associated species. We thank the reviewers for bringing this point to our attention, and we have included the following into the paper, which we hope both adds context to our work by providing a frame of reference, and prevents any confusion:
“Advances in genome sequencing have begun to provide a glimpse into HGT within environmentally, medically and economically important microbiomes [McDaniel et al., 2010; Andam, Carver and Berthrong, 2015]. […] Other natural habitats, such as soil (Coombs and Barkay 2004; Heuer and Smalla 2012) and aquatic environments (McDaniel et al. 2010; Frischer et al. 1994) also show evidence of extensive HGT.”
Reviewer 2 also has made some reasonable requests to add some caveats or temper (tone down) statements in places, e.g. regarding the starting culture as a source of HGT, although we do agree this is an interesting and convincing finding.
We have added additional language to make it clear that these findings are not definitive. See also specific responses to reviewer 2 below.
Reviewer #1:
[…] A major conclusion of the study is that HGT is high in cheese rind communities. However, there is no point of reference on which to base this conclusion. High compared to what?
Please see our second response to the Editor regarding this question.
The authors identified mobile elements and insertion sites indicative of genomic islands for one HGT group (RUSTI). It would be useful to know (and strengthen the study) what other HGT groups identified are associated with mobile elements, and what proportion of the total groups. Also Given bacterial genomes can contain a number of putative genomic islands, what is the proportion identified HGT groups to genomic islands not horizontally transferred among the organisms compared?
We agree that this an important piece of information. Please see our first response to the Editor.
Reviewer #2:
[…] I have the following considerations and a few editing suggestions I would expect the authors will consider in revising their manuscript.
“During the aging of cheese, bacteria and fungi form a multi-species biofilm called the rind [Button and Dutton, 2012]”. Such a statement must be changed. This does not apply to just any cheese. So please amend indicating in what types of cheese this happens.
Thank you for pointing out that this could use some clarification. We have added an additional statement to this sentence to clarify that the formation of multi-species biofilms on the surface of cheese only happens in traditional styles of rinded cheeses: “During the aging of traditional styles of cheese in caves or aging rooms, bacteria and fungi form a multi-species biofilm called the rind”.
Subsection “Iron Acquisition HGT”, sixth paragraph. This is unlikely to play a role in adaptation, at least in the case of cheese. In addition, the authors do not discuss the Staph HTG. I would just leave out this part. Overall it stands as an awkward deviation in a nice story.
Thank you for this comment – we’ve added additional language in an attempt to clarify this point (see below). We agree that genes such as FnBP are not likely to be adaptive in cheese, but their association with this HGT island suggests that it may have been acquired from a pathogenic strain of Staphylococcus. Since we also saw an iron-acquisition HGT island in γ-Proteobacteria that is identical to genes in pathogenic E. coli, our intent was to highlight the convergence of iron acquisition in adaptation to cheese as well as in pathogenesis.
“It is unlikely that these genes could provide a selective advantage to cheese-associated Staphylococci, but Group 31 also contains genes for iron acquisition. […] Since iron acquisition genes may be adaptive both on cheese and in pathogenesis, it is possible that this region was acquired from an animal pathogen, and the virulence genes have been preserved due to their association with these genes.”
Subsection “A potential mechanism of transfer and source of the Actinobacterial RUSTI”, last paragraph. Although this is a convincing initial indication, it should be proven in a remarkably higher number of cases. I recommend the authors to down-tone the statements in this paragraph to make the conclusions more careful and scaled to the only experiment and case reported.
We agree – it was not our intention to suggest that our experiments are a definitive demonstration of the source. We’ve added an additional statement to clarify this:
“Though we have no evidence for direct transmission of ActinoRUSTI from this starter culture, and thus cannot definitively conclude that this was the source, these data are consistent with the hypothesis that HGT from a starter culture could explain some of the dissemination of ActinoRUSTI.”
Discussion, seventh paragraph. This is far too alarming for the readership. HTG may just happily take place naturally in any ecosystem. I suggest rethinking this bit of Discussion.
We agree that this section may be somewhat alarming, we feel that it is nevertheless important to highlight this implication. HGT is known to occur in many environments, and starter cultures and probiotics are not typically screened for mobile elements other than antibiotic resistance plasmids.
Discussion, ninth paragraph. Honestly I do not believe in in vitro models. I know the authors already have experience with this. My opinion is that what happens in reconstituted communities with no cheese around can be very different from what could take place in a real system. I would expect the authors to at least recognize the limitations of in vitro studies of food communities in this part of the Discussion.
As with any model system, there are certainly limitations. Model systems are widely used in biology to address questions that are difficult to address in situ, our intent here is to indicate the ways in which our cheese model system has such utility in the study of HGT. However, we agree that model systems often do not fully encompass the complexity seen in natural systems, but we believe they provide an opportunity to generate predictions about the mechanisms and impacts of processes in more complex systems. To clarify this, we have added an additional statement to this paragraph:
“Having an experimentally-tractable microbial community will allow us to test these ideas under controlled conditions in the laboratory and generate predictions about how these processes work in more complex natural systems.”
Reviewer #3:
[…] I don't have major substantive comments, but did wonder whether the authors had considered doing any analysis of the genomic location of the HGT groups, at least for the large groups. If similar groups are found in very different genomic locations, even among closely related strains, then this would provide evidence for HGT. For instance, this type of analysis would be useful for group 27 and its presence among the B. linens strains (subsection “Functions encoded in HGT regions”, sixth paragraph). Our group has been trying to consider these patterns for HGT in bacteriophage (http://www.sciencedirect.com/science/article/pii/S0042682216302756), and perhaps some consideration of this might help to support or refute the alternatives proposed. However, this is not essential for publication.
Thank you for this suggestion and we agree that this could be an interesting way to look at the data, especially for Group 27 (now reassigned to be Group 29), which appears to contain genes related to core, conserved functions. It appears that Group 29 is in the same genomic location across the genomes. One potential explanation for this could be recombination instead of mobile element-based HGT. Another potential explanation is that mobile elements, such as integrative and conjugative elements, often integrate at conserved sites in the bacterial chromosome, such as at specific tRNAs. Indeed, immediately downstream of Group 29 (less than 200bp) is a tRNA-Leu ORF.