FastQCFastQC Report
Thu 29 Mar 2018
CON-P1.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-P1.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23357502
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG4505791.929054742240844No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG3701201.5845872559488594No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA2777191.1889927270476097No Hit
TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTT1481820.6344085938641898No Hit
GTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC1216860.5209718059747999No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1216320.520740616869047No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAT911230.3901230533984328No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG893610.38257943850331255No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG834320.35719573094759877No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGC788530.33759175103570577No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA696160.2980455701127629No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCA519550.22243388869237815No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAA509640.21819114047384008No Hit
AAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAAGGCGCCCG506180.21670981768512745No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATT484150.2072781584263591No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATA444010.19009310156539855No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAA436070.1866937654548847No Hit
CGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACG411030.1759734409955311No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGG393250.16836132562463227No Hit
GGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCC389720.16685003387776653No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGT365080.15630096060785953No Hit
AAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCT357180.15291874961629032No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGA342380.14658245560676822No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAG332820.14248955217899584No Hit
AAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTT331000.14171035926701409No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTG322630.1381269281278452No Hit
TGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCA320710.13730492241850176No Hit
TGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTT315360.13501443775965427No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG308030.13187625971304637No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCT301510.12908486532506774No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGG280890.12025686650909845No Hit
AGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGC279580.11969601886366102No Hit
AGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAA274270.11742265932375817No Hit
GGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG268220.11483248508337922No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT266010.11388632226168706No Hit
CGGTTTTCCGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTA264570.1132698179796795No Hit
AAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCG263600.11285453384527165No Hit
GGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAA262330.11231081131877886No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG249360.10675799150097472TruSeq Adapter, Index 1 (100% over 49bp)
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT248720.10648398959786025No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCAT245730.10520388695674734No Hit
AGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGAT245080.1049256037738967No Hit
GCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCTCGG243280.10415497342138727No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG239340.10246814920533882No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA236500.10125226576026837No Hit
TAGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAAT234540.1004131349319803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACACG394550.089.992691
AACACGG404550.087.9062962
ACACGGG406100.087.663383
ACGGGAA424550.084.551485
GCGCGGT145050.081.860216
TACCTGG325150.079.8921
CGGGAAA449900.079.886896
GTTGATC342900.079.1695567
ATGTGAG544150.078.761973
GCGACCT155200.078.237672
GTGAGGG561350.078.0907065
CACGGGA458850.078.0876544
TGAGGGC562350.077.972066
GGAAACC476250.075.532938
GGATGTG571300.074.960341
CTGGTTG360200.074.951324
CCTGGTT352850.073.967783
GGGAAAC494850.072.67447
ACCTGGT360000.072.0938952
CGCGGTG169500.070.085917
Produced by FastQC (version 0.11.3)