FastQCFastQC Report
Thu 29 Mar 2018
CON-P2.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-P2.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19996544
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG14164907.0836740588773734No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG3167321.583933703744007No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1389690.6949650899675465No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1293130.6466767457416642No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA763470.381800975208516No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC633130.3166197118862139No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG457630.22885454606556013No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG398270.1991694164751669No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT367720.18389177649897903No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG328800.16442841322980611No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG326270.1631631946000269TruSeq Adapter, Index 2 (100% over 49bp)
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA288320.1441849151533385No Hit
ACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTT265560.1328029483494748No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA252090.126066784340334No Hit
GGGTGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG246580.12331130819405592No Hit
GGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAA241230.12063584587416705No Hit
ATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTAT218550.10929388598349796No Hit
ACAAAATCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGGACAGCTCTT218230.10913385833071956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT418900.084.0027856
CGCGGTG434450.080.992247
GGGCGCG434950.080.950094
GGGCGAT1738550.078.717249
CCGGGCG442300.078.701692
AGGGCGA1762600.077.646768
GGCGCGG456800.077.191225
CGGGCGC453450.077.129133
GAGGGCG1782750.076.80457
CTCCCTC1637100.076.7501194
ATGTGAG1774250.075.5471653
GTGAGGG1828700.074.868365
TGAGGGC1828850.074.848386
GATGTGA1812450.073.874692
GCGGTGG476650.073.7639548
GGATGTG1828300.073.278241
CGGTGGC481750.073.062749
TGTGAGG1880000.072.7328954
GCCGGGC492900.070.6287841
ACTAATC187250.068.453261
Produced by FastQC (version 0.11.3)