FastQCFastQC Report
Thu 29 Mar 2018
CON-P2.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-P2.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19996544
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT5327462.6641903720962983No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC2498581.2495059146220466No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA788490.3943131373101272No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT785490.39281287806532966No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC759920.38002566843550567No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC541740.2709168144255327No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA511390.2557391917323314No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG407920.20399525037926555No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA383160.19161311074553683No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT382350.1912080407494415No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG348760.17441013807185882No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC345870.1729648883327039No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG329520.16478847544855751No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC302190.15112111372845227Illumina Single End PCR Primer 1 (100% over 50bp)
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG276050.13804885484211674No Hit
TGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG264230.13213783341761456No Hit
AGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC236110.11807540342971265No Hit
GATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATG227020.11352961791797622No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG220850.11044408473784269No Hit
TCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTC213360.10669843748999829No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT210450.1052431860225447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA56100.062.5607572
GTATACC938150.060.8425794
CGTCTGG128500.058.925021
ATCGGAA58850.058.9229663
AGATCGG60000.058.3174741
CGTGTCT105200.050.1596071
AGAGCGT72600.046.0868269
AAGAGCG78050.043.4109238
CGGAAGA95000.038.2881935
GTCTGGA1491500.035.4862941
CGCCGTA45050.035.311646-47
TCGGAAG105200.034.7936254
GGAGTCT1664150.034.41255
GAGTCTT1679950.034.1629876
TGGAGTC1666850.034.024264
AGTCTTG1683500.034.012547
TCTGGAG1575750.033.820882
CGTATCA48500.032.17498450-51
GTCTTGG1799700.031.950388
CTGGAGT1756350.031.904983
Produced by FastQC (version 0.11.3)