FastQCFastQC Report
Thu 29 Mar 2018
CON-S11.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S11.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25603258
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG10606554.142656375997149No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1614600.6306228683865155No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1375620.5372831848196818No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG1104480.43138259982381927TruSeq Adapter, Index 19 (97% over 49bp)
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC898790.35104516776732086No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA868020.3390271659958276No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA495440.19350662325864934No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT401880.15696439882768046No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG350880.13704505887492913No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA280590.10959152151651949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT188450.073.534266
GGGCGAT1467800.071.447019
GATCGGA181950.071.410942
GGGCGCG197250.070.3997964
AGGGCGA1496750.070.079148
AGATCGG186100.069.73581
GAGGGCG1520650.068.896827
CGGGCGC201600.068.414263
CGCGGTG208550.066.493627
ATGTGAG1546100.066.4310463
CCGGGCG207200.066.157112
GTGAGGG1588500.065.924585
TGAGGGC1594750.065.742866
GATGTGA1569500.065.410912
GGATGTG1586250.064.559771
TGTGAGG1631800.064.1608664
CTCCCTC1516200.063.5803194
GGCGCGG224050.062.146695
GCCGGGC248250.055.202671
ATCGGAA243000.053.1216243
Produced by FastQC (version 0.11.3)