FastQCFastQC Report
Thu 29 Mar 2018
CON-S12.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S12.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26365901
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT6684892.535430137585664No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC2974151.1280289643809251No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC891540.3381412984900459No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC693440.2630063732697775No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA665490.2524055597417285No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG519360.19698169996162848No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT504080.1911863357144518No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC499810.1895668196584672Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA472630.17925805000936626No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA470440.17842743170430625No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC439140.16655603766395088No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT396460.15036846265940237No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG376550.1428170423608888No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG312820.11864567040587766No Hit
AGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC302590.11476565887128226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA86500.066.215772
GTATACC1161150.062.0606794
ATCGGAA93400.060.924693
AGATCGG94900.060.1161771
CGTCTGG166400.054.8333971
AGAGCGT112600.049.4974379
AAGAGCG115800.048.9416358
CGTGTCT152750.043.2913251
CGGAAGA142250.041.396545
TCGGAAG154450.038.8815084
GTCTGGA1835350.036.1503521
CGCCGTA73200.035.5042746-47
GGAGTCT2067200.035.079125
GAGTCTT2087450.034.7996866
AGTCTTG2092000.034.6878627
TGGAGTC2072650.034.6030274
TCTGGAG1960350.034.19822
CGTATCA76800.032.74287450-51
CTGGAGT2169300.032.675463
GTCTTGG2238200.032.5210238
Produced by FastQC (version 0.11.3)