FastQCFastQC Report
Thu 29 Mar 2018
CON-S3.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S3.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24229804
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG16235846.700772321559019No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG3445541.422025535163223No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1929630.7963869621066683No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA977100.4032636830244273No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC861800.3556776604548679No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG580080.23940763202211623TruSeq Adapter, Index 14 (97% over 49bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA533510.22018750131036968No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT451860.18648933354970595No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG373940.15433059219133594No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA341190.1408141807502859No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA301430.12440463818857139No Hit
GGGTGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG289570.11950984003007206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT401950.084.0989466
GGGCGCG417600.081.1295244
CGCGGTG419250.080.6465457
CGGGCGC423100.079.430483
CCGGGCG423650.078.628942
GGCGCGG438150.077.378065
GGGCGAT2050150.077.119849
CTCCCTC1876850.077.0247594
AGGGCGA2076050.076.164748
GAGGGCG2103650.075.1847847
ATGTGAG2116000.073.184523
GTGAGGG2167450.072.945995
TGAGGGC2171350.072.851986
GCGGTGG469550.072.058178
CGGTGGC470200.071.960489
GATGTGA2152750.071.8833542
TGTGAGG2213950.071.3268364
GCCGGGC468650.071.112441
GGATGTG2176000.071.102471
CTATGCC519750.065.1018894
Produced by FastQC (version 0.11.3)