FastQCFastQC Report
Thu 29 Mar 2018
CON-S6.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S6.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22816311
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG10929404.790169629086841No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2918021.2789184018398065No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG1475360.6466251270856187TruSeq Adapter, Index 16 (97% over 49bp)
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1244670.5455176342924147No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1038840.4553058555346655No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA647470.2837750589917888No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC559950.24541653556528048No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG329710.14450627009773842No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT321050.1407107397861118No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG256660.11248970089862467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT352600.081.439286
GGGCGCG369650.077.9789664
CGCGGTG372100.077.200477
GGGCGAT1399100.076.580799
CGGGCGC377300.075.912173
CCGGGCG376050.075.426992
AGGGCGA1425350.075.159398
GAGGGCG1450400.073.883677
GGCGCGG393700.073.251285
GATCGGA256900.072.3501742
ATGTGAG1472850.071.418443
CTCCCTC1372250.071.3796694
AGATCGG260650.071.3055951
TGAGGGC1510800.070.9620746
GTGAGGG1511000.070.934175
GATGTGA1497500.070.186332
GGATGTG1524350.068.9460751
TGTGAGG1568150.068.26814
CGGTGGC423400.067.936839
GCGGTGG424750.067.675618
Produced by FastQC (version 0.11.3)