FastQCFastQC Report
Thu 29 Mar 2018
GF-P2.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-P2.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19781093
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3974662.0093227406594774No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1813470.9167693615312359No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC562910.28456971513151474No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA562190.2842057311999898No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC466920.23604357959390818Illumina Single End PCR Primer 1 (100% over 50bp)
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC441960.22342546996771107No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA377180.1906770267952332No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC370830.1874668907324787No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG366220.18513638250424283No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA311800.15762526367981788No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT311580.1575140463674075No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG266830.1348914339566575No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG261880.13238904442742372No Hit
GATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATG221210.11182900762864822No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG217670.1100394199653174No Hit
AGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC201160.10169306620215576No Hit
TGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG200350.10128358427919024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA78550.065.853992
ATCGGAA82100.062.6120573
AGATCGG85200.061.265751
GTATACC763250.056.52289294
AGAGCGT91550.055.5409289
CGTCTGG108000.055.3421631
AAGAGCG96800.052.4802178
CGGAAGA119000.044.5087435
CGTGTCT91000.043.046511
TCGGAAG125700.042.65174
CGCCGTA63850.037.05844546-47
CGTATCA68950.033.87968450-51
GTCGCCG73250.032.8156644-45
GTCTGGA1214100.032.6983031
GCCGTAT73450.031.7690448-49
GGAGTCT1371450.031.6841665
GAGTCTT1378350.031.5386966
CCGTATC75600.031.518348-49
AGTCTTG1380650.031.4077137
TCGCCGT73150.031.35119646-47
Produced by FastQC (version 0.11.3)