FastQCFastQC Report
Thu 29 Mar 2018
GF-S1.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S1.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13036850
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG5796194.44600497819642No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1616861.240222906607041No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA830440.6369943659703072No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC740480.5679899669015138No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG485100.3720990883533982TruSeq Adapter, Index 21 (97% over 49bp)
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA441520.33867076786186845No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT253360.19434142450055036No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG207280.15899546286104388No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA175670.13474880818602653No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA154640.11861761084924656No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA148740.11409197774002156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT199700.080.665746
GGGCGCG204850.078.43134
CGCGGTG208550.077.2200857
CGGGCGC210950.075.516543
CCGGGCG210700.074.5174562
GGCGCGG217050.074.3913045
CTCCCTC748600.073.9063594
GGGCGAT791300.073.7994469
AGGGCGA804750.072.577428
GAGGGCG819350.071.261227
GATCGGA100550.070.283642
AGATCGG101800.069.553121
CGGTGGC235500.068.503219
ATGTGAG834800.068.4124453
GCGGTGG235900.068.327038
GTGAGGG854100.068.276695
TGAGGGC855600.068.253026
GCCGGGC234100.067.047221
GATGTGA831700.066.426552
TGTGAGG878450.066.330564
Produced by FastQC (version 0.11.3)