FastQCFastQC Report
Thu 29 Mar 2018
GF-S10.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S10.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21318541
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3877971.819059756481459No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1643660.7710002293308909No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT677520.3178078649941382No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC491360.23048481601062662No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA408680.191701674143648No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC394340.1849751350244841No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA347510.16300834095541528No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG334770.15703232224006325No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT281540.13206344655574695No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA261460.12264441548790792No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC233070.10932736907277096No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC230800.10826256824986288Illumina Single End PCR Primer 1 (100% over 50bp)
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG219350.10289165661008415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC689700.062.30271594
GATCGGA51350.057.8288842
ATCGGAA56500.052.4797673
AGATCGG56900.052.194041
CGTCTGG100800.051.1867871
AGAGCGT69700.040.65279
AAGAGCG74050.039.787478
CGTGTCT97450.037.4675451
GTCTGGA1106200.036.2607541
GGAGTCT1239200.034.7906845
GAGTCTT1241800.034.7464876
AGTCTTG1253400.034.384887
TGGAGTC1252550.034.105354
TCTGGAG1198150.033.6860542
CGGAAGA92650.033.172485
CTGGAGT1289800.032.876663
CGCCGTA40850.032.44113546-47
GTCTTGG1349300.032.136828
TCTTGGA1393750.031.0951149
TCGGAAG104650.029.94974
Produced by FastQC (version 0.11.3)