FastQCFastQC Report
Thu 29 Mar 2018
GF-S2.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S2.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30963933
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG16955295.475819237820984No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG4504821.4548604016162934No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1191220.38471211005397793No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1093950.3532981420674176No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC894670.28893939280904657No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG723230.23357174942860134No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA573270.1851412092901764No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG545320.17611457820942836No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT507970.16405215706932322No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA456710.14749741255414808No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT419990.13563845393929772No Hit
GGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAA352340.1137904542036052No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG338800.10941762469257378TruSeq Adapter, Index 22 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT521200.085.5435946
GGGCGAT2121700.082.011599
CGCGGTG550500.081.110237
AGGGCGA2158800.080.563138
GGGCGCG557500.080.4032754
GAGGGCG2184400.079.603917
ATGTGAG2165050.078.551873
GTGAGGG2233150.077.676975
TGAGGGC2243950.077.449376
CCGGGCG577850.076.1032942
GGCGCGG591100.075.762735
GATGTGA2180750.075.471862
CGGGCGC596700.074.6480263
GGATGTG2213800.074.3466341
CTCCCTC2230700.073.427394
TGTGAGG2398000.072.404344
GCGGTGG624350.071.5614248
CGGTGGC628600.071.055059
GCCGGGC746150.058.9788061
CTATGCC768400.058.0908294
Produced by FastQC (version 0.11.3)