FastQCFastQC Report
Thu 29 Mar 2018
GF-S3.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S3.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21067081
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG9248944.390233274367721No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2878331.3662690146774485No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1322930.627960750708653No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC957320.45441511332300855No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA714620.3392116829094643No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT441130.20939303361486103No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG432490.20529184845304388TruSeq Adapter, Index 7 (97% over 36bp)
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG342180.1624240206794667No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA325790.15464411040143625No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG298550.14171398496070717No Hit
TCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTT251310.11929037534910507No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA246270.11689801733804508No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA238510.11321454547974634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT354600.082.666856
GGGCGCG362500.080.9588554
CGCGGTG368650.079.5927357
CGGGCGC371350.078.4086763
CCGGGCG367950.078.0384142
GGCGCGG381700.076.899955
GGGCGAT1274250.073.948069
CTCCCTC1211550.073.3670894
AGGGCGA1300050.072.494988
GCCGGGC400700.071.639781
GAGGGCG1319000.071.428697
GCGGTGG413250.071.1503458
CGGTGGC413550.071.0532769
ATGTGAG1341250.068.739583
GTGAGGG1373850.068.513465
TGAGGGC1382800.068.197666
GATGTGA1341900.066.435032
TGTGAGG1417150.066.3992164
GGATGTG1359800.065.630181
CTATGCC464650.063.291594
Produced by FastQC (version 0.11.3)