FastQCFastQC Report
Thu 29 Mar 2018
GF-S3.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S3.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21067081
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3362471.5960777860017723No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1444510.6856716409833902No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA558300.2650106106299207No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC515020.24446671088415145No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC463210.21987384013950487Illumina Single End PCR Primer 1 (100% over 50bp)
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC372930.17702025259218399No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC369570.17542534725147732No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA351040.16662963416716345No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG295510.14027097536673447No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA261470.12411306530790858No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG231010.10965448891566895No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT219610.10424320293827133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA77750.068.591082
AGATCGG83600.063.6904371
ATCGGAA84600.062.4893463
GTATACC687250.055.17434794
AGAGCGT96200.054.4620179
CGTCTGG106450.052.447091
AAGAGCG101500.052.220138
CGGAAGA119150.045.791265
TCGGAAG134750.040.6270034
CGTGTCT99600.039.634241
CGCCGTA66200.037.37468746-47
CGTATCA68600.035.07969350-51
GTCGCCG75900.033.61857244-45
GTCTGGA1038250.033.0016561
GAGTCTT1167400.032.694086
GGAGTCT1164900.032.6768955
AGTCTTG1176900.032.3986937
TGGAGTC1187500.031.736454
TCGCCGT75600.031.67084146-47
CCGTATC78400.031.591148-49
Produced by FastQC (version 0.11.3)