FastQCFastQC Report
Thu 29 Mar 2018
GF-S4.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S4.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20971509
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT2435231.1612087618492308No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1033420.49277331450016304No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT796480.379791458974173No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC416850.19876967365581563Illumina Single End PCR Primer 1 (100% over 50bp)
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC321890.15348919336228975No Hit
TCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTC244350.116515220721599No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA238290.11362558602721436No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC223020.106344278802255No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA219350.10459428551374153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA80850.062.9372862
ATCGGAA84500.060.1694033
AGATCGG84050.060.0485841
GTATACC461800.058.7574494
AGAGCGT90650.054.8413969
AAGAGCG111300.045.4263428
CGGAAGA115000.045.3586735
CGTCTGG79450.044.8344231
TCGGAAG129900.040.7677544
CGCCGTA64150.036.92181846-47
GTCTGGA752350.033.9856071
GGAGTCT814900.033.52215
GAGTCTT822600.033.3151866
CGTATCA69450.033.2291850-51
AGTCTTG828200.032.9835327
TGGAGTC835500.032.4400024
GGTCGCC76150.030.82454144-45
GTCTTGG900500.030.473328
TCTGGAG837050.030.4682852
GCCGTAT77150.030.0629148-49
Produced by FastQC (version 0.11.3)