FastQCFastQC Report
Thu 29 Mar 2018
GF-S5.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S5.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28789430
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG9029423.136366367795403No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT3020791.0492705135183296No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2131410.7403446334296997No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1788160.621116847398507No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1041370.36171956165856706No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA936930.32544235853228076No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG717200.24911920798709805No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC639000.22195646110395378No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA622700.21629466092242886No Hit
GTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC507400.1762452400064885No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATG497850.17292805032958278TruSeq Adapter, Index 25 (100% over 49bp)
TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTT412750.14336859048616107No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGC337290.11715758179304No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA336240.11679286460343258No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG329480.11444478060176946No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGA310100.10771314333072937No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT309920.10765062038393954No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG305330.10605628524079844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT362250.082.360966
CGCGGTG387650.077.037557
ATGTGAG1231050.074.569023
TGAGGGC1276900.073.665916
GTGAGGG1283450.073.134565
GAGGGCG1327550.070.976067
GGATGTG1265450.070.297741
GGGCGAT1363350.069.177689
AGGGCGA1376450.068.406758
GCGGTGG439400.068.00738
GCGACCT151850.067.918552
GATGTGA1353650.065.748462
CTCCCTC1347800.065.61690594
CTAATCC266000.065.605132
ATCCTAG273200.064.698985
CGGTGGC467400.063.913049
GATCCTT543250.060.7937287
GATCGGA119050.059.1636542
GAGATCC562250.058.72475
AGATCGG120750.058.40381
Produced by FastQC (version 0.11.3)