FastQCFastQC Report
Thu 29 Mar 2018
GF-S5.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S5.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28789430
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3631881.261532444372813No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1494940.5192669670778477No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT1008030.35013892251427No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA680850.236493046232593No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA659080.2289312431680655No Hit
GCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCG591150.20533577774898634No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC482490.16759275887018255Illumina Single End PCR Primer 1 (100% over 50bp)
GTCCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC438480.1523058983800652No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC420520.1460674976892561No Hit
TCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTC351340.1220378451396919No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG349830.12151334708606597No Hit
CCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTA330020.114632349442139No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA320590.11135684172975985No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG312760.10863709354440154No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA311980.10836616077497888No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC309860.10762977940167624No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC309360.10745610454948222No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA289950.10071404678731047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA89200.064.101382
AGATCGG96750.059.8367271
GTATACC680200.059.10344394
ATCGGAA102150.055.752173
AGAGCGT101250.055.644919
CGTCTGG105500.045.0305021
CGGAAGA134050.043.714535
AGTTTAC207100.041.1789494
TTCGCGC55250.039.888038
TCGGAAG147300.039.714914
GTCTGGA1032000.037.0685961
GGAGTCT1137500.035.5736475
CGCCGTA74950.035.5519746-47
GAGTCTT1147000.035.3186266
GTCCCGT203350.035.2167171
AGTCTTG1153050.035.0853847
TGGAGTC1154250.034.87924
CGTTCGA58600.034.400268
TCTGGAG1131250.033.7240032
CGTATCA77050.033.61102350-51
Produced by FastQC (version 0.11.3)