FastQCFastQC Report
Thu 29 Mar 2018
GF-S6.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S6.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23179624
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG12609125.4397431123127795No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG1244850.5370449494780416No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1200540.5179290224897523No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG944440.4074440551753557No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA905400.39060167671399676No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC735170.3171621765737011No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT686180.29602723495428573No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA647380.2792883957047793No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT420520.18141795570109334No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG399610.1723971018684341No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG337000.14538630997638272TruSeq Adapter, Index 1 (97% over 37bp)
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA316270.13644311055261293No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG307430.13262941624937488No Hit
ATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTAT238130.1027324688269318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT267550.082.726976
GGGCGAT1618650.078.889059
GGGCGCG282350.078.383794
CGCGGTG283050.078.163757
AGGGCGA1656900.077.096248
CTCCCTC1566350.076.7374794
GAGGGCG1674350.076.306777
ATGTGAG1681450.074.669693
GTGAGGG1717650.074.2345055
TGAGGGC1721850.074.152446
GATGTGA1678200.072.4452062
GGCGCGG309050.071.826455
GGATGTG1696900.071.640871
CGGGCGC308600.071.304393
TGTGAGG1789200.071.30134
CCGGGCG310650.069.975872
CGGTGGC333550.066.357819
GCGGTGG336950.065.813748
GCCGGGC381500.057.0131071
ACTAATC157700.056.3634451
Produced by FastQC (version 0.11.3)