FastQCFastQC Report
Thu 29 Mar 2018
GFR-P1.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-P1.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22953732
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3512681.5303306669259709No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1747380.761261828795422No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC1282230.5586150435144925No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC1130200.4923818052768064No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT860110.3747146651359352No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC791180.3446846900538875No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG783610.3413867514006001No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG736000.32064502626413866No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC732010.3189067468418643No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC649920.28314349927933286No Hit
ATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTC572120.24924922883999864No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA512970.22347999880803696No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT504550.2198117500021347No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC499680.21769009065715325No Hit
GCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTC497310.21665757881986247No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG489020.21304596568436016No Hit
GTCCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC467350.2036052350876973No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT449720.19592456686346257No Hit
GTCCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT449710.19592021027343176No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG448850.19554554353078618No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA444430.19361993073718906No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC438590.1910756821592236No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC416100.18127771118003816No Hit
GGCGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTT404640.17628505900478406No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG391520.1705692128844233No Hit
GCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGC390330.17005077867076257No Hit
GCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAG387370.16876122802165677No Hit
GCGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTT384860.16766772392393534No Hit
CCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATA381010.1659904367620917No Hit
TCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC370500.16141166063976No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG357030.1555433338683226No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC351890.15330404659251054Illumina Single End PCR Primer 1 (100% over 50bp)
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC339080.14772325476310344No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA327660.1427480289479724No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA323470.1409226177250828No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC319550.1392148344330238No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA295360.1286762431486087No Hit
GGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGG295220.12861525088817802No Hit
GCTGGGTAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC288020.1254785060660288No Hit
GCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCC280700.1222894821635105No Hit
GGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAG277830.12103914082468158No Hit
GTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTACT271080.11809844255391672No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA269950.11760614788044053No Hit
TCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG263460.1147787209504755No Hit
GGTGTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAAT259740.11315806945903176No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT258970.11282261202666302No Hit
GCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATT254650.11094056513337351No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA250430.10910208414039164No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG247750.10793451801214722No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT246730.10749014582900941No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA246280.10729409927762508No Hit
GTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTC244540.10653605261227238No Hit
GGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTC236270.10293315265683158No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC230600.1004629661093891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA57700.068.0753562
GTATACC551550.066.338894
AGATCGG59950.065.8110351
CGTCTGG78300.055.9131361
AGAGCGT68800.055.5326849
AGTTTAC135450.051.52776794
TTACGAC65800.049.7698482
ATCGGAA79900.048.3978163
CGGAAGA84150.047.526025
CGTGTCT73000.046.7670171
GTCCCGA47250.046.576841
CATAGTT182050.045.72163494
GTACGTA72300.044.3580061
GTTACGA75350.043.3113941
TCGGAAG92450.043.2510154
ATCCCCA328800.042.38252394
TACGTAT75250.042.333352
CGTATAT82050.041.6894764
TACGACT81500.041.5671583
TTCGCGC76000.040.625828
Produced by FastQC (version 0.11.3)