FastQCFastQC Report
Thu 29 Mar 2018
GFR-P2.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-P2.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17433523
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG11649436.682200723284675No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2166521.2427321775409366No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1111290.6374443077282773No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA967160.5547702549851801No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG836740.47996036142551335No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA769990.4416720590554187No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGC766170.43948087830554966No Hit
GTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC634040.36369011587617717No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG609890.34983749411980586No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA587770.3371492956415063No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC439590.2521521324175268No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAT414840.23795534614546926No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC398130.22837036438360736No Hit
TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTT295950.16975914736224No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT277990.1594571561926984No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG243120.13945546175606618No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG237070.13598513622289654No Hit
GGGTGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG219620.12597568489168826No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA207990.11930462936263657No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAA200710.11512876657231014No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATT198410.11380946926217952No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA197650.11337352754231031No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATA196710.11283433646773519No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGA179080.10272163578182104No Hit
TGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCA177090.10158015680479499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT325750.083.966476
CGCGGTG342900.079.823517
ATGTGAG1467300.077.1450653
TGAGGGC1506350.076.878316
GTGAGGG1507600.076.84775
GGATGTG1497550.075.554711
CTCCCTC1403500.075.4981494
CAACACG103250.075.121071
GAGGGCG1552750.074.690197
GCGGTGG368200.074.403938
AGGGCGA1559900.074.21988
GGGCGAT1599050.072.662729
CGGTGGC376600.072.633529
AACACGG108250.072.172832
TGTGAGG1650250.070.139364
ACACGGG112100.069.736033
GATGTGA1661700.068.057732
ACGGGAA118400.067.176785
GTTGATC143450.065.4808967
CTAATCC120150.062.129422
Produced by FastQC (version 0.11.3)