FastQCFastQC Report
Thu 29 Mar 2018
GFR-P3.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-P3.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19212251
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG11487765.979393044573486No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2556001.3304011070852655No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1309730.6817160571137656No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC904080.4705747390037742No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA665920.34661216949539125No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG608630.3167926548534058TruSeq Adapter, Index 11 (100% over 49bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA546260.2843289940361491No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT510400.26566382044456943No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG371610.19342345673081202No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG355800.18519433251210385No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA328390.17092739419238276No Hit
ATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTAT275090.14318467940065951No Hit
TCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTT243670.12683053120636412No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA218430.11369308052450493No Hit
GGGTGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG196140.10209110842867918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT345400.082.9807746
GGGCGCG358950.079.956854
CGCGGTG360300.079.584347
CGGGCGC365550.077.9473653
CCGGGCG364550.077.439232
GGGCGAT1450600.076.844729
CTCCCTC1342300.076.1605394
AGGGCGA1474200.075.6167458
GGCGCGG382400.075.065945
GAGGGCG1498650.074.47037
ATGTGAG1502600.072.7260743
GTGAGGG1541650.072.338495
TGAGGGC1543700.072.309096
GATGTGA1530800.071.279032
GCGGTGG405150.070.879328
TGTGAGG1578150.070.674364
CGGTGGC406500.070.610169
GGATGTG1551200.070.3744661
GCCGGGC403250.069.966941
GATCGGA104850.065.9552462
Produced by FastQC (version 0.11.3)