FastQCFastQC Report
Thu 29 Mar 2018
GFR-P4.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-P4.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25868955
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG16624596.42646368977796No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2439810.9431420789900481No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA2073940.8017100033611717No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1134130.4384135346789231No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1034440.3998769954178667No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC909910.3517382128501132No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT621900.24040399003361365No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG576950.22302794991138994TruSeq Adapter, Index 12 (100% over 49bp)
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG470100.18172361427046435No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA414740.1603234456127045No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA371480.14360069821142757No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG341060.13184142923438538No Hit
ATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTAT333020.12873345676313558No Hit
TCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTT284680.11004696556161622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT335700.079.743146
CTCCCTC1915800.077.67258594
GGGCGAT2133700.076.006099
GGGCGCG354900.075.445084
CGCGGTG355400.075.421857
AGGGCGA2166950.074.866448
GAGGGCG2195950.073.870477
ATGTGAG2198800.072.329983
GTGAGGG2255600.071.8991855
TGAGGGC2261800.071.697386
CCGGGCG367750.071.619532
CGGGCGC375050.070.939943
GATGTGA2246500.070.741882
GGCGCGG381950.070.372765
TGTGAGG2304450.070.2954944
GGATGTG2269800.070.042321
GATCGGA123150.064.622852
GCGGTGG417750.064.188248
CGGTGGC419150.063.930369
AGATCGG125000.063.5155031
Produced by FastQC (version 0.11.3)