FastQCFastQC Report
Thu 29 Mar 2018
GFR-S10.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S10.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20111009
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT2206311.0970657911793487No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC806340.4009445771716377No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC483360.24034597170137012Illumina Single End PCR Primer 1 (100% over 50bp)
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT479820.23858574176959493No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC324790.161498610039904No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC291440.14491565291428193No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG212900.1058624159533716No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA208440.10364472513537237No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC206450.10265521734886598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA80950.070.28652
AGATCGG86600.066.1966861
ATCGGAA87500.064.81933
AGAGCGT94000.059.1866239
AAGAGCG98250.057.0569658
GTATACC509150.050.53666794
CGGAAGA116300.050.4682275
CGTCTGG73900.047.1159631
TCGGAAG127150.045.713494
CGCCGTA67800.038.78058646-47
GTCGCCG75850.035.43712644-45
CGTATCA73350.034.762650-51
TCGCCGT75300.033.8570246-47
GGTCGCC78400.033.41541744-45
GTCTGGA712400.033.203481
GCCGTAT77300.033.10608348-49
GGAGTCT796750.032.610685
GAGTCTT807000.032.212916
CCGTATC81350.032.20895848-49
GAGCGTC87100.032.07286510-11
Produced by FastQC (version 0.11.3)