FastQCFastQC Report
Thu 29 Mar 2018
GFR-S3.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S3.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21092959
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG10572665.012411961735667No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG3680451.744871357309328No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1336780.6337565061402717No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1277470.6056381183882261No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA730010.34609179300068804No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC590180.27979952931212737No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT571430.2709103070839895No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATG467810.22178490936240855TruSeq Adapter, Index 7 (97% over 36bp)
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG340580.16146620301115647No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG334310.15849364709806718No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT298350.14144530409412923No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA244990.11614776286247937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT449250.083.827886
GGGCGCG459500.082.083974
CGCGGTG464800.081.023597
CCGGGCG461850.079.2169652
GGCGCGG482150.078.3262255
CGGGCGC478900.077.962873
GGGCGAT1434800.075.365469
CTCCCTC1362450.074.6474394
AGGGCGA1460650.074.0027168
GCGGTGG509900.073.949328
CGGTGGC512100.073.5306859
GAGGGCG1477500.073.177847
GCCGGGC504800.072.326571
ATGTGAG1494000.071.275733
GTGAGGG1526550.070.6998445
TGAGGGC1529600.070.639076
GATGTGA1493700.068.9800952
TGTGAGG1572450.068.704274
GGATGTG1511550.068.196381
ACTAATC184850.067.841311
Produced by FastQC (version 0.11.3)