FastQCFastQC Report
Thu 29 Mar 2018
GFR-S4.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S4.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28006991
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG10433653.7253734255136512No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1946750.6950943069892799No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1290020.4606064250172394No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC918000.32777530438739383No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA695850.24845582304789546No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG651610.2326597669846075No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATG610980.21815267480894324TruSeq Adapter, Index 12 (97% over 37bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA416910.14885926160364746No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT403940.14422827500462296No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG326640.11662802333888707No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG282680.1009319423139744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT326150.079.9937446
CGCGGTG342050.076.247817
GGGCGCG341350.076.203364
GGGCGAT1410800.075.587579
AGGGCGA1441350.073.995258
CGGGCGC349550.073.810063
GAGGGCG1468550.072.624737
CCGGGCG350450.072.470022
GGCGCGG365050.071.4891745
CTCCCTC1409700.070.81502594
GATCGGA181300.069.3150562
TGAGGGC1552050.068.7659766
GTGAGGG1552050.068.689235
ATGTGAG1523550.068.6637343
AGATCGG187200.067.245761
GATGTGA1515650.066.7782
TGTGAGG1611600.066.152964
GGATGTG1546000.065.490711
CGGTGGC400550.065.217459
GCGGTGG400500.065.143478
Produced by FastQC (version 0.11.3)