FastQCFastQC Report
Thu 29 Mar 2018
GFR-S4.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S4.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28006991
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4052221.4468601785889816No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1469430.5246654308561745No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC896620.3201414961000273Illumina Single End PCR Primer 1 (100% over 50bp)
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC555500.19834333506230642No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT551440.19689369700586543No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA486210.17360308360151935No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC456510.16299858845957427No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG381390.1361767138783313No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC377520.13479491602650212No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA340840.12169818599934566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA147650.072.4226152
ATCGGAA153400.069.22573
AGATCGG155600.068.974261
AGAGCGT167900.062.3819739
AAGAGCG171700.061.712698
CGGAAGA200350.054.3916325
GTATACC839200.053.8344594
TCGGAAG213150.051.6272284
CGTCTGG124600.048.5733831
CGCCGTA126950.038.44307746-47
CGTATCA129950.036.16827850-51
GTCGCCG139250.035.92394344-45
GTCTGGA1163050.035.590061
GGAGTCT1307050.034.6797335
GAGTCTT1314100.034.4639056
CCGTATC142600.034.16060648-49
AGTCTTG1325350.034.126737
TCGCCGT137350.033.9925446-47
GCCGTAT139000.033.87885348-49
TGGAGTC1325950.033.8028224
Produced by FastQC (version 0.11.3)