FastQCFastQC Report
Thu 29 Mar 2018
GFR-S8.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S8.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26113252
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4689491.795827651033276No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1761750.6746574497883298No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC658850.25230484506487355No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA545890.20904711523482408No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT538470.2062056460834522No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC428740.16418483611309692No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG422950.16196757110144688No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC409230.1567135338026838Illumina Single End PCR Primer 1 (100% over 50bp)
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC391520.14993153667723957No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA389910.1493149914840174No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT295230.11305753875465224No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG289070.1106985832327586No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG289030.11068326533975928No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA283750.10866130346385047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA78200.064.345712
ATCGGAA83600.059.6339263
AGATCGG84900.058.887291
GTATACC911650.057.1028294
CGTCTGG129000.053.3261
AGAGCGT95150.051.1634989
AAGAGCG102900.048.2690668
CGGAAGA128800.040.3881845
GTCTGGA1306700.036.6641461
CGTGTCT116250.036.6203841
TCGGAAG146250.035.3405764
GGAGTCT1473800.035.3315735
GAGTCTT1487900.034.979296
CGCCGTA64150.034.7979546-47
AGTCTTG1495750.034.78187
TGGAGTC1486050.034.7172854
TCTGGAG1416600.033.9252322
CGTATCA66150.032.7904750-51
CTGGAGT1562550.032.651283
GTCTTGG1617400.032.3157168
Produced by FastQC (version 0.11.3)