FastQCFastQC Report
Thu 29 Mar 2018
CON-P3.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-P3.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25826399
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4406321.70613022744673No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC2182260.8449726189082728No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC2169490.8400280658561807No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC1845140.7144395159387107No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG1198580.46409102562072246No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC1070130.4143550945681587No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG899220.3481786214175658No Hit
GTCCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC876950.3395556616313409No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT698230.27035515094458196No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC591200.2289130590757155No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT588420.22783664110509558No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA547130.21184912383642798No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC532440.20616114542333216No Hit
GCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGC495210.19174566303262025No Hit
GCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCC490650.18998002780023648No Hit
ATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTC469410.18175588474413332No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA463210.17935524034922562No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG418510.16204736866335875No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA400060.15490351558496404No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC394120.1526035433743589No Hit
GCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCT393090.15220472664423715No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC392460.15196079019765785No Hit
CCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATA389200.150698515886787No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC382580.14813524719415974No Hit
CGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCA364200.14101849816538498No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA357620.13847071750111195No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG350670.13577967257456217No Hit
TCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC333540.1291469244318575No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC332860.12888362794983535No Hit
GCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATT331580.12838801104249958No Hit
GGGTGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC330190.12784980205718963No Hit
GGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGG327450.12678887211492396No Hit
GCTGGGTAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC321220.12437661169875057No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG320890.12424883546482807No Hit
GTCGGGGAAAAAAAGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG318680.12339312189825612No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT316300.12247158421117865No Hit
GCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAG312910.12115897380815654No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT311280.12052783665272111No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG299940.11613698061429314No Hit
GCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTC293390.11360081597128581No Hit
GTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGC291460.11285351860319356No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA287070.11115370749131537No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC270790.10485007995113838No Hit
CGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCG270030.10455580741240773No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA269330.10428476691620849No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT269330.10428476691620849No Hit
GGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGT264650.10247266759876202No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA264360.10236037939319377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC689500.066.6643694
GATCGGA39650.057.350182
CGTCTGG98400.053.386021
AGATCGG44500.051.0454031
TTACGAC74950.050.9625932
CATAGTT214700.046.23288394
GGGCGGT409750.045.9218181
ATCCCCA526650.045.60242594
GTCCCGA50600.043.404611
GTTACGA90250.042.3655431
CGTGTCT84650.042.112571
GTACGTA48350.041.825531
GTCCCGT290700.041.124441
TACGACT97250.041.1162763
GTTACGG207500.040.5381859
AGAGCGT53750.040.47929
TTCGCGC48950.040.224198
TCCCGTT337350.039.9142572
TCGCGCC49700.039.4283879
AGTTACG217400.038.8215458
Produced by FastQC (version 0.11.3)