FastQCFastQC Report
Thu 29 Mar 2018
CON-P4.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-P4.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21968673
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG12292625.595522314889024No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG3279261.4926982617475346No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1328740.6048339833725961No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1132880.5156797590823988No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC738460.3361422877021293No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA726990.33092121677080816No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG688670.3134781968851737TruSeq Adapter, Index 4 (100% over 49bp)
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT547000.24899091538209886No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG415860.1892968227985368No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA399430.1818179914644822No Hit
ATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTAT291800.13282550111242494No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA244040.11108545336352359No Hit
TGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGA230540.10494033936414822No Hit
TCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTT228320.10392980950647314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT387550.080.738826
GGGCGCG400950.078.0912864
CGCGGTG402600.077.7588357
GGGCGAT1546200.076.930319
CTCCCTC1438600.075.9479794
AGGGCGA1571200.075.713148
CGGGCGC413500.075.3801353
CCGGGCG416000.074.531642
GAGGGCG1597350.074.5285347
GGCGCGG425650.073.802685
ATGTGAG1612600.072.336673
GTGAGGG1648600.072.202675
TGAGGGC1650800.072.12566
GATGTGA1638950.071.139272
GGATGTG1657450.070.314041
TGTGAGG1700850.069.9210364
GCGGTGG450000.069.621418
CGGTGGC452700.069.218449
GATCGGA135800.068.812922
AGATCGG137450.067.984591
Produced by FastQC (version 0.11.3)