FastQCFastQC Report
Thu 29 Mar 2018
CON-S10.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S10.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16681665
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG10636936.376419859768194No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1962911.1766870992793586No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1224980.7343271789716433No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA632810.37934462776947026No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC581840.34879012376762153No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA349800.2096912988002097No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG304780.18270358504381906No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT278370.16687183203834868No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA222220.1332121224110423No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA216890.13001699770376637No Hit
GGGTGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG185000.11090020090920182No Hit
ATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTAT173600.10406635069101315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT250600.078.911126
GGGCGCG252600.078.346864
GGGCGAT1367850.076.8406759
AGGGCGA1385850.075.856418
CGGGCGC259350.075.691263
CCGGGCG259650.075.024472
CTCCCTC1286250.075.00342694
CGCGGTG263600.074.980047
GAGGGCG1401950.074.968297
ATGTGAG1401750.073.475633
GTGAGGG1447650.072.574955
TGAGGGC1451200.072.4675756
GGCGCGG274750.072.201725
GATGTGA1425900.072.1685642
GGATGTG1439600.071.396561
TGTGAGG1474950.071.1679154
GCCGGGC289550.067.229271
GCGGTGG303350.065.186078
CGGTGGC305500.064.653129
GGGATGT206900.057.4813421
Produced by FastQC (version 0.11.3)