FastQCFastQC Report
Thu 29 Mar 2018
CON-S10.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S10.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16681665
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4019382.4094597271915004No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1702911.020827357461021No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC451670.27075834456572534No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC447800.2684384322548139No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC428140.2566530379311658No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA388800.23307026007296033No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG348470.20889401627475435No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA339300.20339696307293065No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA299490.17953243875836133No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT273360.1638685347056184No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG263330.1578559454347033No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG251250.15061446204560516No Hit
AGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC197700.11851334983648215No Hit
GCCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT191630.11487462432556941No Hit
GATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATG173040.10373065278555828No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG168340.10091318822191908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC797750.055.30903694
CGTCTGG87600.051.4888571
GATCGGA27000.046.2878532
CGTGTCT83850.042.4611321
AGATCGG31250.039.432111
ATCGGAA31750.039.218093
GTCTGGA1114550.036.1727371
GGAGTCT1274950.034.9560935
TGGAGTC1267950.034.793884
GAGTCTT1284050.034.7562646
AGTCTTG1292050.034.5220537
TCTGGAG1192500.034.1514932
CTGGAGT1350550.032.2761153
GTCTTGG1401100.031.9250268
TCTTGGA1440700.031.1089329
AGAGCGT40800.029.0260039
GTTCGAT74300.028.8406246
GGTTCGA76150.027.9575185
GTGTCTG615950.027.89351
AAGAGCG43800.027.3602948
Produced by FastQC (version 0.11.3)