FastQCFastQC Report
Thu 29 Mar 2018
CON-S12.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S12.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26365901
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG17017366.454306264747031No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2814401.0674393414433287No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1710800.6488684001354629No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA919920.34890520145698795No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA896670.34008699342381665No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC661000.25070260257747307No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG522270.19808539825739313TruSeq Adapter, Index 20 (97% over 49bp)
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG431940.16382523775690427No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG378500.14355663400238056No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT354680.13452223764323473No Hit
GGGTGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG312420.11849395929993062No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA308630.11705649657108247No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA288610.10946335571843345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT337900.083.424146
GGGCGCG352700.080.140854
CGCGGTG358350.078.784947
GGGCGAT2126200.078.462229
AGGGCGA2157350.077.323198
CTCCCTC1998050.076.6297694
GAGGGCG2187450.076.260347
CGGGCGC370700.075.323773
CCGGGCG369900.074.85142
ATGTGAG2188100.074.5685353
TGAGGGC2254850.073.956296
GTGAGGG2257300.073.85695
GATGTGA2233400.072.982542
GGCGCGG389000.072.9161765
GGATGTG2266100.071.909731
TGTGAGG2319050.071.831514
GCGGTGG416050.067.8482368
CGGTGGC418800.067.494029
GCCGGGC427300.064.66871
CTATGCC483550.058.2358894
Produced by FastQC (version 0.11.3)