FastQCFastQC Report
Thu 29 Mar 2018
CON-S7.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S7.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26512891
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG10747534.0536997643900845No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG4320781.6296902514327842No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1307470.4931450138726856No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA801400.3022680551887005No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG725180.27351977571966785TruSeq Adapter, Index 3 (97% over 37bp)
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA617880.2330488968554957No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC537520.20273911283382864No Hit
GTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC505420.19063179492572124No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG481470.18159845337122987No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA426280.1607821644195648No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC350780.132305450959686No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT349420.13179249294239545No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT330170.12453187394765813No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGC315760.11909678201445478No Hit
GGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAA302850.11422745260032185No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG298800.11269989379883168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT495950.085.696476
CGCGGTG517500.082.159117
ATGTGAG1374750.075.505423
GAGGGCG1408600.074.969887
GCGGTGG568750.074.863748
GTGAGGG1415100.074.5982745
TGAGGGC1420050.074.336796
CGGTGGC579200.073.547019
GGATGTG1440600.071.9476241
GGGCGAT1472350.071.837579
AGGGCGA1470750.071.776848
GATGTGA1511000.068.641322
CTCCCTC1433850.067.4279794
CGGGCGC643700.066.023163
TGTGAGG1622050.065.089324
GATCGGA141900.064.432222
AGATCGG144250.063.320741
CTAATCC207850.062.7820172
ATCCTAG219250.059.9890985
GGGCGCG714250.059.982284
Produced by FastQC (version 0.11.3)