FastQCFastQC Report
Thu 29 Mar 2018
CON-S7.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCON-S7.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26512891
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4213501.5892269160688663No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1858250.7008854673750969No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT1122690.42345061502346165No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1056650.3985419771838537No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC692450.2611748375535508Illumina Single End PCR Primer 1 (100% over 50bp)
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC510350.19249126773839942No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG459650.17336849459381853No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC395960.14934621803408765No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA368000.13880040467861463No Hit
TGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG364250.13738599838093854No Hit
GTCCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC349270.13173591669048842No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG347640.1311211214197652No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT345040.13014046638670979No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA303930.11463480161405257No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG303390.11443112710718722No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT293650.11075744248335649No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG289730.10927891643351907No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA284680.10737418261931525No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA273740.10324788798022819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA108650.069.31432
AGATCGG117000.064.748251
ATCGGAA116850.063.973223
GTATACC748000.060.25618794
AGAGCGT123950.059.334519
CGGAAGA150250.050.575295
CGTCTGG108450.047.5225141
TCGGAAG167150.046.2961434
CGCCGTA91850.038.2465746-47
CGTGTCT106500.037.9281731
TTCGCGC53900.037.3163078
CGTATCA93700.036.46885750-51
GTCCCGT166650.036.3436851
GTCTGGA1202350.035.4336361
TCGCGCC57200.035.4095849
ACGTTCG40500.034.691037
AACCTGG347200.034.49088794
GGAGTCT1324000.034.329795
GAGTCTT1338450.034.0297476
AGTCTTG1343750.033.874427
Produced by FastQC (version 0.11.3)