FastQCFastQC Report
Thu 29 Mar 2018
GF-P1.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-P1.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24056726
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4413261.834522287031078No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1952370.811569288356196No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC652430.27120481814524555No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA641930.2668401344389091No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC632710.2630075264605832Illumina Single End PCR Primer 1 (100% over 50bp)
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC463270.19257400196518845No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC455530.18935660654737474No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT424330.1763872606771179No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA417440.1735231967974362No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG379450.15773135546374847No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA325490.13530103805480428No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG325270.1352095875390525No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG287390.11946347146324068No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT278250.11566411821791545No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG270110.11228044913509842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA101350.070.323992
AGATCGG103500.069.2530751
ATCGGAA104950.067.379853
AGAGCGT116900.059.6151779
CGTCTGG126350.055.165481
GTATACC881900.054.62944494
CGGAAGA146150.049.5197375
AAGAGCG144850.048.2092678
TCGGAAG164200.044.7835044
CGTGTCT111200.039.0806351
CGCCGTA86250.038.46933446-47
CGTATCA90150.036.02840850-51
GTCTGGA1338250.033.176391
GGTCGCC100150.033.1528844-45
GCCGTAT100350.032.4803248-49
CCGTATC103350.032.37879648-49
GAGTCTT1502900.032.27866
GGAGTCT1506150.032.207135
AGTCTTG1517850.031.898697
TGGAGTC1517450.031.685864
Produced by FastQC (version 0.11.3)