FastQCFastQC Report
Thu 29 Mar 2018
GF-P2.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-P2.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19781093
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG10938625.529835990357054No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG3315321.6760044553655353No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1463440.7398175621539214No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA802830.40585724964742853No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC713770.36083445945074927No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG527810.26682549846967507No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG505330.255461111274286TruSeq Adapter, Index 6 (100% over 49bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA494080.2497738623442092No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT367890.18598062301208532No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA278870.14097805414493525No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA260760.13182284720060716No Hit
ATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTAT201090.10165767887547973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT391950.083.074396
GGGCGCG405750.080.242024
CGCGGTG406600.080.1623157
CGGGCGC407850.079.0103153
CCGGGCG408750.077.84732
GGCGCGG426550.076.505485
CTCCCTC1312050.075.1833894
GGGCGAT1441550.074.990529
AGGGCGA1465600.073.7703558
GAGGGCG1494150.072.38767
CGGTGGC454000.071.889929
GCGGTGG453850.071.880268
GCCGGGC444000.071.4948651
ATGTGAG1498700.070.762183
GTGAGGG1539750.070.271045
TGAGGGC1540500.070.193086
GATGTGA1529400.069.301792
TGTGAGG1573600.068.654874
CTATGCC474750.068.5906394
GGATGTG1552750.068.280781
Produced by FastQC (version 0.11.3)