FastQCFastQC Report
Thu 29 Mar 2018
GF-S1.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S1.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13036850
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT2043951.5678250497627877No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC873890.6703229691221423No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC555960.4264527090516498Illumina Single End PCR Primer 1 (100% over 50bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA310700.23832444187054388No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC270580.20755013672781383No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC257420.1974556737248645No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC230150.1765380440827347No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA192780.14787314420277906No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG183910.14106935340975774No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG165150.1266793742353406No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA161340.12375688912582411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA87250.073.618132
ATCGGAA89400.071.065573
AGATCGG91000.070.130741
AGAGCGT95800.066.21869
AAGAGCG99450.064.165528
CGGAAGA113100.057.008325
TCGGAAG122650.053.138874
GTATACC440000.051.67629294
CGTCTGG58000.048.939441
CGCCGTA75700.039.605946-47
CGTGTCT59000.038.949951
CGTATCA79700.037.0927550-51
GTCGCCG82300.036.80024344-45
CCGTATC85500.035.53659448-49
TCGCCGT81200.035.36065346-47
GCCGTAT83050.035.33997348-49
TCGTGTA89900.035.02314814-15
GGTCGCC84700.034.62013644-45
GAGCGTC93850.033.92195510-11
TGGTCGC90000.033.39166642-43
Produced by FastQC (version 0.11.3)