FastQCFastQC Report
Thu 29 Mar 2018
GF-S11.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S11.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30526754
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG12642444.14142951458252No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2193160.7184386522065201No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1475440.4833268548631145No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC961720.3150416844188544No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA822250.2693538919991297No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG815830.267250818740833No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA776250.25428514279638115No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG413900.13558598467429586No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT403990.13233965196561678No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG391700.12831367527644766No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATG354650.11617678053814696TruSeq Adapter, Index 7 (97% over 35bp)
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT352760.11555765149481666No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG334450.10955963414911393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT365250.082.290096
GGGCGCG384200.078.090854
CGCGGTG388850.077.417127
GGGCGAT1708650.076.644939
AGGGCGA1746250.074.9498
GAGGGCG1782150.073.484167
CGGGCGC406250.073.2614443
CCGGGCG407250.072.03372
GGCGCGG422100.071.325065
ATGTGAG1799250.071.137223
CTCCCTC1733350.071.0662294
GTGAGGG1854250.070.4870455
TGAGGGC1858950.070.404826
GATGTGA1812750.068.295612
TGTGAGG1928350.067.8363654
GGATGTG1845100.067.1452941
CGGTGGC462900.065.103579
GCGGTGG463600.065.056068
GCCGGGC489250.059.9624331
CTATGCC536200.056.23010694
Produced by FastQC (version 0.11.3)