FastQCFastQC Report
Thu 29 Mar 2018
GF-S11.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S11.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30526754
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4841191.5858843033229146No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC2080700.6815988362208442No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC671290.21990218809376194No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT527740.17287786313605435No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC490790.1607737265481944No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA478360.15670188844840824No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA457450.1498521591912458No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG396440.12986641160733958No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC362270.1186729516017327No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT348860.1142800836276271No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC344180.11274700218699965Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA341610.11190511772067217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC930300.058.85476394
GATCGGA78800.057.6573032
ATCGGAA86100.052.775313
AGATCGG87400.052.4739461
CGTCTGG153400.049.501841
AGAGCGT103200.043.02639
AAGAGCG111200.040.522458
GTCTGGA1460750.034.426621
CGTGTCT135000.034.35491
CGGAAGA142050.034.041275
GGAGTCT1627400.033.692545
GAGTCTT1643800.033.426996
AGTCTTG1649700.033.1801267
TGGAGTC1651700.032.8474354
CGCCGTA62250.032.42391246-47
TCTGGAG1601000.031.5303482
CTGGAGT1720450.031.1338043
GTCTTGG1792050.030.753378
TCGGAAG162500.030.1889614
CGTATCA65050.030.0938250-51
Produced by FastQC (version 0.11.3)