FastQCFastQC Report
Thu 29 Mar 2018
GF-S12.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S12.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20066984
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG7020303.4984330480355195No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1147920.5720441098672326No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA689300.34349955130277676No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA558910.2785221735363919No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC450830.2246625601535338No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG388620.19366138927504004No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT334230.1665571667371639No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA294580.14679834298965902No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT267450.13327862323506112No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG231790.11550814013705298No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG227500.11337030019060164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT186400.081.428276
GGGCGAT929600.079.277419
AGGGCGA953700.077.234858
CGCGGTG199650.076.188957
GAGGGCG970450.075.916497
GGGCGCG202200.075.38634
ATGTGAG989050.072.554533
TGAGGGC1022500.072.0933466
GTGAGGG1022000.071.9288565
CGGGCGC212950.071.182853
GATGTGA989150.070.028272
CCGGGCG213400.069.275632
GGCGCGG221850.068.794775
GGATGTG1011350.068.475231
TGTGAGG1078400.068.118164
CTCCCTC1020750.067.93703594
GCGGTGG246900.061.6082888
CGGTGGC250800.060.706329
GCCGGGC269600.054.8492051
CTAATCC130950.049.871522
Produced by FastQC (version 0.11.3)