FastQCFastQC Report
Thu 29 Mar 2018
GF-S4.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S4.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20971509
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG6224402.968026764311524No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA1454910.693755513730557No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1228830.5859521124588603No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA591750.2821685363699865No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC580660.27688040951178094No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG427590.20389090742111118TruSeq Adapter, Index 22 (97% over 37bp)
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT379060.18074998799561826No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT364840.1739693600493889No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA304240.14507301310554238No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG271610.12951380847224678No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA214670.1023626864428306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT164800.077.190136
GGGCGAT839350.076.432999
AGGGCGA863450.074.2729648
GAGGGCG879800.072.855487
CGCGGTG180150.070.613187
GTGAGGG924100.069.3003545
ATGTGAG903000.069.250993
TGAGGGC930900.068.906536
GGGCGCG189850.067.135854
GATGTGA904600.066.860232
CTAATCC217600.065.8872
CTCCCTC918050.065.77534594
GGATGTG921750.065.612991
ACTAATC221550.064.7494051
TGTGAGG1006650.063.5600974
CGGGCGC203900.061.9098173
CCGGGCG202400.060.9316142
GGCGCGG209650.060.8413735
ATCCTAG237550.060.7665675
GATCGGA104700.059.2317962
Produced by FastQC (version 0.11.3)