FastQCFastQC Report
Thu 29 Mar 2018
GF-S6.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S6.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23179624
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4965522.142191780160023No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC2129300.9186085158240703No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT773770.33381473314666366No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC659370.284461042163583No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC473120.2041102996321252No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA428480.18485200622753847No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG405130.1747785037410443No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT337880.14576595375317564No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA325750.1405329094207913No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC324130.13983401974078613Illumina Single End PCR Primer 1 (100% over 50bp)
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC315950.13630505827014278No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA280250.12090360050706604No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG270580.11673183309617102No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT236710.10211986182347048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC881400.062.20539594
GATCGGA67100.060.357682
ATCGGAA72000.055.472093
AGATCGG72950.055.0735361
CGTCTGG126750.052.7915231
AGAGCGT85200.045.279839
AAGAGCG91300.043.12958
CGTGTCT107750.040.731631
CGGAAGA107800.038.4921575
GTCTGGA1360100.037.4438481
GGAGTCT1520500.036.1384745
GAGTCTT1530900.035.9071586
TGGAGTC1522750.035.7756164
AGTCTTG1540900.035.5991977
TCTGGAG1459300.035.1645772
CGCCGTA52550.035.0561946-47
CTGGAGT1574150.034.2616423
TCGGAAG125100.033.2405664
GTCTTGG1670100.033.022958
TCTTGGA1704600.032.351839
Produced by FastQC (version 0.11.3)