FastQCFastQC Report
Thu 29 Mar 2018
GF-S7.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S7.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21992041
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG12569695.715563189428393No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG1476930.6715747756199618No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1283810.5837611888773762No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1281120.5825380190951809No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA909220.41343138638200977No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC874800.39778026968938446No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA695290.3161552854507683No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT446960.2032371620260257No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG417890.19001874359910478No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG344650.1567157864065459No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA304090.1382727505828131No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT274870.12498612566246126No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG258580.11757890047585852No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTATCTCGTATG249240.11333190948489046TruSeq Adapter, Index 27 (97% over 49bp)
TCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTT243140.11055817875203125No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA230290.10471515581477864No Hit
ATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTAT228380.10384665979842435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT326000.082.969646
CGCGGTG342900.079.058787
GGGCGCG343500.078.639644
GGGCGAT1644050.078.262079
AGGGCGA1672750.076.908068
CTCCCTC1580100.076.6037794
GAGGGCG1699500.075.708597
CGGGCGC354550.075.6047443
ATGTGAG1690750.074.441213
CCGGGCG357450.074.060642
TGAGGGC1744250.073.717576
GTGAGGG1745250.073.522145
GGCGCGG369850.073.2669145
GATGTGA1687050.072.190422
TGTGAGG1796600.071.427634
GGATGTG1711300.071.200551
GCGGTGG394250.068.916538
CGGTGGC395300.068.5670559
GCCGGGC416850.063.402191
CTATGCC455550.059.53147594
Produced by FastQC (version 0.11.3)