FastQCFastQC Report
Thu 29 Mar 2018
GF-S8.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S8.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26439947
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG12409344.693405777250613No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2343540.8863633501232057No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA985550.3727503689776685No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG848200.32080245849206884No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA825230.312114846523709No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC641770.2427274154520809No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG474190.17934604785705507No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA419200.15854797288360675No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT412570.1560404035605669No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG391390.1480297974878694No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA372110.14073780102509284No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT355950.13462583718492324No Hit
GGACAGCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAA352990.13350631905578328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT368800.085.114766
GGGCGAT1548950.081.720999
CGCGGTG388000.080.9758767
AGGGCGA1583350.079.918658
GAGGGCG1615700.078.356317
GGGCGCG402700.077.945334
ATGTGAG1608550.077.077513
TGAGGGC1658100.076.3246
GTGAGGG1661650.076.070665
GATGTGA1626400.073.87132
CTCCCTC1629600.072.87794
GGATGTG1654800.072.6607361
GGCGCGG433350.072.5962145
CCGGGCG426150.072.028052
CGGGCGC435100.071.686963
TGTGAGG1805300.070.014094
CGGTGGC456350.068.940489
GCGGTGG458100.068.6769948
CTATGCC534850.058.60428694
CTAATCC176000.054.3736532
Produced by FastQC (version 0.11.3)