FastQCFastQC Report
Thu 29 Mar 2018
GF-S9.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGF-S9.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24742381
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4758211.9231010952422083No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1858780.7512534868814768No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC815250.32949537071634294Illumina Single End PCR Primer 1 (100% over 50bp)
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT729310.2947614459578486No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC661760.2674601122664791No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC556900.22507938908547243No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC442540.17885910010035008No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG401330.162203467806918No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA395610.15989164502801895No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA391420.1581981944259932No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG373110.1507979365445872No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA296770.11994399407235708No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG290500.11740988064164075No Hit
GCCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT248900.10059662406782921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA128400.074.682842
AGATCGG134250.071.926171
ATCGGAA135150.070.787323
AGAGCGT149500.063.2062879
CGGAAGA176550.054.857125
GTATACC985000.053.6602994
CGTCTGG127150.053.1043131
TCGGAAG194950.050.39874
AAGAGCG190600.049.9217458
CGCCGTA112000.039.56599846-47
CGTGTCT108800.038.3075261
GTCTGGA1300700.037.2958261
CGTATCA116850.036.88560550-51
GGAGTCT1461800.036.1295475
GAGTCTT1475100.035.865986
TGGAGTC1464600.035.81384
AGTCTTG1492600.035.3679057
TCTGGAG1400550.034.7405172
GGTCGCC127350.034.11281644-45
CTGGAGT1561700.033.217373
Produced by FastQC (version 0.11.3)