FastQCFastQC Report
Thu 29 Mar 2018
GFR-P2.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-P2.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17433523
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4519242.5922700764498376No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC2116051.213782205696462No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA767200.4400716940574777No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC639380.3667531800657848No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC605090.3470841779943159No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT580040.3327153094644152No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC533590.3060712398750384No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC531620.30494123304853527No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC451450.2589551176775916No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA418350.2399687085622338No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC370480.21251011628573296No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG349330.20037831710779286No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG331980.19042622652919894No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG326860.187489355995343No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA326400.18722549653331688No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT324600.18619300298625815No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG323980.18583736632004902No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC300790.17253540778877569No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC278190.15957187769792713No Hit
TGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG267360.15335970818979042No Hit
GGCGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTT261760.1501475060433855No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA248830.14273076073034693No Hit
CCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATA245890.14104435460348433No Hit
GCGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTT231880.13300811316221053No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC226100.12969266166109972No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG225630.12942306612381216No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG219890.12613055892374708No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG205880.11809431748247329No Hit
AGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC200060.1147559216803167No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG199060.11418231415417296No Hit
GTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTACT188610.10818811550597088No Hit
ATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTC185580.10645008470175535No Hit
GCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTC183290.10513652346688619No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT180960.10380001793097128No Hit
GATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATG180890.1037598654041412No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC180790.10370250465152682No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT179240.10281341298600402No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA177380.10174650298737668No Hit
GTCCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT176060.10098934105286694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC761300.064.1170194
CGTCTGG104150.062.6298031
CGTGTCT84750.051.5513341
GATCGGA23050.044.0282552
AACCTGG202100.043.09132894
GGGCGGT174000.041.1096531
AGATCGG25450.041.023151
GTTACGG93650.040.6952749
GCGCCGA15250.038.5481951
ACGTTCG117900.038.3000537
AGTTACG101750.037.548028
TTACGAC22000.037.373522
GTTCCGT164850.037.1152341
AGACGTT117400.036.9476625
GTCTGGA1237350.036.6812061
GTCCCGA34650.036.3743671
CGAAGTT106600.036.0619745
TTACGCC54000.035.853739
AGTTTAC87000.035.7077994
GCAGACG118150.035.434093
Produced by FastQC (version 0.11.3)