FastQCFastQC Report
Thu 29 Mar 2018
GFR-P3.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-P3.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19212251
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4195682.1838565402877568No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1886570.9819619783231024No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC639610.33291778251283516No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC556490.2896537214717838Illumina Single End PCR Primer 1 (100% over 50bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA526950.274278115562825No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC432910.22533018124737178No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC412300.21460265119376173No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT403110.20981924502235577No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA397100.20669103271657238No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG326750.17007377219879127No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA309130.16090254077983887No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG279140.145292709323858No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT265360.13812020257282712No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG238590.12418638503109293No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG223610.11638927682133654No Hit
AGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC203250.10579187207162763No Hit
GCCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT197380.10273652993602884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA82650.072.083342
AGATCGG87750.067.634441
ATCGGAA87950.066.997683
AGAGCGT99050.058.499179
GTATACC792550.056.9225794
CGTCTGG111100.056.298041
AAGAGCG104600.056.159888
CGGAAGA123750.049.370245
TCGGAAG135600.045.7070544
CGTGTCT96850.043.701721
CGCCGTA71250.037.6961846-47
CGTATCA72000.036.4594750-51
GTCTGGA1186700.034.818171
GGAGTCT1333300.034.074615
GAGTCTT1339900.033.9346286
AGTCTTG1349800.033.6613667
TGGAGTC1340900.033.581744
GTCGCCG83200.033.04324344-45
GGTCGCC81000.033.0124244-45
TCTGGAG1275450.032.6678662
Produced by FastQC (version 0.11.3)